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Items: 3

1.

Dissecting the yeast heat shock response by CRISPRi/a

(Submitter supplied) CRISPRi screens on the repression of 129 protein kinases and 161 transcription factors in S. cerevisiae. We quantify perturbation effects on cellular fitness at 23, 30 and 38°C, expression of the SSA1 Hsp70 chaperone (as proxy for heat shock response activity) and thermotolerance. The integration of these phenotypes allowed us to identify core signaling pathways of the HSR and their contributions to temperature-associated growth and heat resistance.
Organism:
synthetic construct
Type:
Other
Platform:
GPL19424
84 Samples
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Series
Accession:
GSE155455
ID:
200155455
2.

Illumina NextSeq 500 (synthetic construct)

Organism:
synthetic construct
108 Series
1081 Samples
Download data
Platform
Accession:
GPL19424
ID:
100019424
3.

beforeHS_TF_MinusATC_Rep2

Organism:
synthetic construct
Source name:
Saccharomyces cerevisiae transformed with Plasmid-encoded DNA barcodes
Platform:
GPL19424
Series:
GSE155455
Download data
Sample
Accession:
GSM4704752
ID:
304704752
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Supplemental Content

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