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Items: 3

1.

Detection of Q-modification in RNAs from S. pombe and S. flexneri via reverse transcription using RT-KTq I614Y (Q-MaP-Seq)

(Submitter supplied) In most eukaryotes and bacteria, queuosine (Q) replaces the guanosine at the wobble position of tRNAs harboring a GUN anticodon. To faithfully detect Q-modification in RNAs from Schizosaccharomyces pombe and Shigella flexneri, Q-MaP-Seq was established and applied to tRNAs from S. pombe WT (AEP1) cells and Shigella flexneri WT cells and tgt∆ cells. Q-modification of in vitro-transcribed RNAs and RNAs isolated from S. more...
Organism:
Shigella flexneri; Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing; Other
Platforms:
GPL16192 GPL30040
30 Samples
Download data: XLSX
Series
Accession:
GSE216921
ID:
200216921
2.

Illumina MiSeq (Schizosaccharomyces pombe)

Organism:
Schizosaccharomyces pombe
57 Series
381 Samples
Download data
Platform
Accession:
GPL16192
ID:
100016192
3.

in vitro-transcribed tRNA Asp, 3% Q

Organism:
Schizosaccharomyces pombe
Source name:
mixing of unmodified and Q-modified tRNA-Asp to 75% Q-modification RT via RT-KTq I614Y using 6.25 µM dCTP
Platform:
GPL16192
Series:
GSE216921
Download data: XLSX
Sample
Accession:
GSM6697783
ID:
306697783
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