GSM7461921[Accession] - GEO DataSets

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Items: 3

Select item 2002343011.

Identification of pan-phylum conserved parasitic nematode proteins that activate immunity

(Submitter supplied) A cocktail of five bioinformatically and experimentally prioritized recombinant proteins was used immunize pigs and test for active antibody responses.

Organism:: Ascaris suum; Sus scrofa

Type:: Protein profiling by protein array

Platform:: GPL33467
111 Samples

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Accession:: GSE234301

ID:: 200234301

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Select item 1000334672.

Ascaris Suum and Trichuris Suis IgG antibody profiling with Ascaris Suum protein microarray

(Submitter supplied) Cloning and expression was performed using standard techniques. All sequences were PCR amplified from cDNA using sequence specific forward and reverse primers that encompassed the mature protein and contained Sac I (forward) and Xho I (reverse) restriction sites for downstream subcloning. Translation stop sites were incorporated into all reverse primers. Amplified sequences were first cloned into pCR2.1-TOPO by TA cloning then transformed into DH5α cells for sequence verification. more...