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Items: 3

1.

Dissecting primary (translation independent) from secondary (translation dependent) IFN-mediated differential gene expression

(Submitter supplied) NIH-3T3 cells were pretreated for 15 min with either DMSO (mock) or cycloheximide followed by addition of either mock, 100 U/ml IFNalpha or 100 U/ml IFNgamma for 1h. During the last 30 min, 500 µM 4-thiouridine was added to cell culture medium. Total cellular RNA was isolated using Trizol reagent and nascent RNA was purified as described (Dölken et al. RNA 2008) . Three replicates of nascent RNA were analyzed by Affymetrix Mouse Gene ST 1.0 arrays
Organism:
Mus musculus
Type:
Expression profiling by array
Platform:
GPL1261
18 Samples
Download data: CEL
Series
Accession:
GSE30457
ID:
200030457
2.

[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 07, 2012: annotation table updated with netaffx build 32 June 23, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site All probe sets represented on the GeneChip Mouse Expression Set 430 are included on the GeneChip Mouse Genome 430 2.0 Array. more...
Organism:
Mus musculus
601 DataSets
4546 Series
40 Related Platforms
58941 Samples
Download data
Platform
Accession:
GPL1261
ID:
100001261
3.

Mock, + CHX, Replicate 3

Organism:
Mus musculus
Source name:
Mock, + CHX
Platform:
GPL1261
Series:
GSE30457
Download data: CEL
Sample
Accession:
GSM755535
ID:
300755535
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