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Items: 4

1.

Diverse and specific gene expression responses to stresses in cultured human cells.

(Submitter supplied) TT3 and TN are cell cycle time courses and should be centered or transformed before clustering or computing fourier scores. The preferred method is SVD centering preceded by KNNImpute missing-value estimation, although mean-centering is a viable alternative. Hela Heat 42 degrees time course has 2 zeroes (the second is labeled "Cold 25 degrees 0h"). Normalization is by centering or zero transformation (average 2 zeroes) WI38 Crowding (1-3 days) was by plating cells at ~50%? confluence and allowing to age. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
10 related Platforms
174 Samples
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Series
Accession:
GSE4301
ID:
200004301
2.

Cell division cycle in a human cancer cell line (HeLa)

(Submitter supplied) This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
6 related Platforms
114 Samples
Download data
Series
Accession:
GSE3497
ID:
200003497
3.

SHBA

(Submitter supplied) Material is transferred from microtiter plate wells to the slide surface by an automated print head
Organism:
Homo sapiens
4 Series
70 Samples
Download data
Platform
Accession:
GPL3001
ID:
100003001
4.

"0 hr Hela Double Thymidine, exp3"

Organism:
Homo sapiens
Source name:
Hela Reference 3 primed with oligo dT (channel 1) "0 hr Hela Double Thymidine, exp3" (channel 2)
Platform:
GPL3001
Series:
GSE3497 GSE4301
Download data
Sample
Accession:
GSM79634
ID:
300079634
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