GEO DataSets

(Submitter supplied) TT3 and TN are cell cycle time courses and should be centered or transformed before clustering or computing fourier scores. The preferred method is SVD centering preceded by KNNImpute missing-value estimation, although mean-centering is a viable alternative. Hela Heat 42 degrees time course has 2 zeroes (the second is labeled "Cold 25 degrees 0h"). Normalization is by centering or zero transformation (average 2 zeroes) WI38 Crowding (1-3 days) was by plating cells at ~50%? confluence and allowing to age. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

10 related Platforms

174 Samples

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(Submitter supplied) This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

6 related Platforms

114 Samples

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(Submitter supplied) Material is transferred from microtiter plate wells to the slide surface by an automated print head

Organism:

Homo sapiens

4 Series

70 Samples

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