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Items: 3

1.

Analysis of transcription in the Drosophila melanogaster testis

(Submitter supplied) Identification and annotation of all the genes in the sequenced Drosophila genome is a work in progress. Wild-type testis function requires many genes and is thus of potentially high value for the identification of transcription units. We therefore undertook a survey of the repertoire of genes expressed in the Drosophila testis by computational and microarray analysis. We generated 3141 high-quality testis expressed sequence tags (ESTs). more...
Organism:
Drosophila melanogaster
Type:
Expression profiling by array
Platform:
GPL5
8 Samples
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Series
Accession:
GSE462
ID:
200000462
2.

Dmel.testis.1.0

(Submitter supplied) The array was assembled using 1,681 testes ESTs, representing 1,527 Drosophila melanogaster EST sequences and 144 anonymous clones from the bs collection (CLONE_ID). An aliquot of the same DNA preparation used in the sequencing reactions (GB_ACC), diluted 2/50 in TE, provided material for the PCRs. Inserts were PCR amplified (1X PCR buffer [Life Technologies], 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM T7 22-mer primer, 1 µM T3 20-mer primer, 2 µl of plasmid DNA dilution [approximately 10 ng], 0.035 U/µl recombinant Taq DNA polymerase [Life Technologies], in a volume of 100 µl, cycled 94C 2 min, 30X[94 C 0.5 min, 55 C 0.5 min, 2.5 min 72 C], 10 min 72 C in a PTC-225 DNA Engine Tetrad [MJ Research]), and successful amplification was confirmed by agarose gel electrophoresis (PCR-QC: passed = product present, failed = no product). more...
Organism:
Drosophila melanogaster
1 Series
8 Samples
Download data
Platform
Accession:
GPL5
ID:
100000005
3.

ovary b

Organism:
Drosophila melanogaster
Source name:
y w[67c1] ovary
Platform:
GPL5
Series:
GSE462
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Sample
Accession:
GSM8
ID:
300000008
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Supplemental Content

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