GEO DataSets

(Submitter supplied) Post-transcriptional modifications of tumor suppressors, including microRNA-mediated downregulation, are important events in tumor progression. To identify microRNAs involved in oncogenic transformation, we examined global microRNA profiles of three ras transgenic zebrafish models. Four microRNAs are upregulated in all transgenic systems. Analysis of the predicted targets shows that three of them target Jmjd6, and overexpression of Jmjd6 in ras transformed melanocytes blocks proliferation and melanoma development. more...

Organism:

Danio rerio

Type:

Non-coding RNA profiling by array

Platform:

GPL15403

19 Samples

Download data: TXT

(Submitter supplied) Custom-designed Agilent zebrafish 8x15k miRNA platform. The 15k design contained a duplicate of 7604 probes of 60-oligonucleotide length. The probes consisted of 2x22 nucleotide sequences antisense to mature miRNAs separated by a spacer of 8 nucleotides (CGATCTTT) and with a second spacer with the same sequence at the end. From 7604 probes 546 were designed for left (5') and right (3') arms of the hairpins of zebrafish miRNAs known in miRBase, while the remainder 7058 probes corresponded to predicted hairpin structures in the zebrafish genome that might include additional miRNAs but were not considered in this study Protocol: the arrays were produced according to manufacturer's protocol, check website: http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/default.aspx

Organism:

Danio rerio

3 Series

28 Samples

Download data: TXT