GSM998217[Accession] - GEO DataSets

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Items: 3

Select item 2000406221.

Expanded Genomic Profiling of Circulating Tumor Cells in Metastatic Breast Cancer Patients to Assess Biomarker Status and Biology Over Time (CALGB 40502 and CALGB 40503, Alliance).

(Submitter supplied) We developed a novel approach to isolate tumor cells with high purity from blood which was subjected to immunomagnetic enrichment using EpCAM beads followed by fluorescence activated cell sorting (IE/FACS) to isolate EpCAM-positive cells away from leukocytes (CD45+). Duplicate samples of 20 cells were isolated from the same enriched blood from MBC patients and then subjected to DNA and RNA profiling in parallel. more...

Organism:: Homo sapiens

Type:: Expression profiling by RT-PCR; Genome variation profiling by genome tiling array

Platforms:: GPL6359 GPL16020
275 Samples

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Accession:: GSE40622

ID:: 200040622

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000160202.

Applied Biosystems TaqMan TLDA 64-Breast cancer associated-gene panel

(Submitter supplied) We selected 64 genes from previously published data focused on QPCR analyses of CTCs. Included in the list are clinically relevant genes, stem cell and epithelial-mesenchymal transition (EMT) markers, and highly expressed genes in breast and prostate cancers. We also included EPCAM and PTPRC (encodes CD45) to measure the expression of these markers specific for epithelial cells and leukocytes, respectively. more...