txid10245[Organism:noexp] - GEO DataSets

Select item 2000105491.

Identification of Vaccinia Virus HLA Class I Peptides Presented by the Immune System

(Submitter supplied) Seeking to identify HLA class I peptides that originate from vaccinia virus proteins to understand the mechanism of immune protection. Note that vaccinia-infected B cells will still continue to present (primarily) a wide variety of peptides originating from endogenous proteins; this data set contains evidence for more than 5000 such peptides. The objective and challenge is to detect and identify the peptides that originate from the pathogen (vaccinia virus) in the presence (background) of this large number of endogensous 'self' peptides. more...

Organism:: Homo sapiens; Vaccinia virus; Bos taurus

Type:: Protein profiling by Mass Spec

Platform:: GPL6461
3 Samples

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Series

Accession:: GSE10549

ID:: 200010549

Select item 2000112342.

Monkeypox and Vaccinia infection of cultured human cells

(Submitter supplied) We analyzed effects of monkeypox (MPX) and vaccinia (VAC) infection on cultured human cells. Our custom-designed arrays contain >18,000 human genes as well as genes for each open reading frame of MPX and VAC. A reference experiement design type is where all samples are compared to a common reference. Compound Based Treatment: Chemical used for treating infected cells Infection: Virus used for infection Cell Line: Cell line used for infection Time: Time cells were harvested after infection Keywords: reference_design

Organism:: Homo sapiens; Monkeypox virus; Vaccinia virus; Variola virus

Type:: Expression profiling by array

Platforms:: GPL6763 GPL6764 GPL6762
265 Samples

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Series

Accession:: GSE11234

ID:: 200011234

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000333133.

Illumina HiSeq 2500 (Vaccinia virus)

Organism:: Vaccinia virus

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Platform

Accession:: GPL33313

ID:: 100033313

Select item 1000064614.

2D-LC MS/MS Analyses of HLA Class I Peptides Isolated from B-cells Infected with Vaccinia Virus

(Submitter supplied) B-cell cultures were infected with live vaccinia virus (NYBOH strain). Cells were lysed, HLA/peptide complexes were enriched by immunoaffinity (W6/32 mAb), peptides were released with acid and separated from HLA proteins by 10 kDa ultrafilitration. HLA class I peptides were separated and identified by 2-dimensional liquid chromatography and tandem mass spectrometry to identify class I peptides derived from vaccinia proteins, that were being presented to the immune system.