GEO DataSets

Analysis of primate livers exposed to ciprofibrate at various doses for 4 or 15 days. Peroxisome proliferator-activated receptor-α (PPARα) agonists such as ciprofibrate cause hepatocarcinogenesis in rodents but not in primates. Results identify species differences in response to PPARα agonist.

Organism:

Macaca fascicularis; Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 5 dose, 2 time sets

Platform:

GPL8300

Series:

GSE2853

23 Samples

Download data: CEL

(Submitter supplied) Liver gene expression was examined in male cynomolgus monkeys treated with ciprofibrate (PPAR-alpha agonist) for 4 days at 400 mg/kg/day and treated for 15 days at 0, 3, 30, 150 or 400 mg/kg/day. The untreated control group were given only the vehicle (0.5% hydroxypropyl methylcellulose). Two animals per group were used for the 4 day treatment and four animals per group were used for the 15 day treatment (except the 15 day control group, which had three animals). more...

Organism:

Homo sapiens; Macaca fascicularis

Type:

Expression profiling by array

Dataset:

GDS1442

Platform:

GPL8300

23 Samples

Download data: CEL

(Submitter supplied) Samples and RNA preparation: The series is composed of 4 hybridizations for analysis of differentially expressed genes in the liver of ciprofibrate dosed vs. control rats. Rats (200-250 g) were given (by gastric intubation) tap water (control group, n=5) or ciprofibrate (50 mg/kg body weight, once daily for 60 days, n=4). Liver total RNA extraction was performed first by ultracentrifugation on a cesium chloride cushion and then using TRIZOL Reagent (GIBCO BRL Life Technologies, New York, NY). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL264

4 Samples

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(Submitter supplied) In rodents, treatment with peroxisome proliferator-activated receptor alpha (PPARalpha) agonists results in peroxisome proliferation, hepatocellular hypertrophy and hepatomegaly. Drugs in the fibrate class of PPARalpha agonists have also been reported to produce rare skeletal muscle toxicity. Although target-driven hepatic effects of PPARalpha treatment have been extensively studied, a characterization of the transcriptional effects of this nuclear receptor/transcription factor on skeletal muscle responses has not been reported. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL3631

484 Samples

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(Submitter supplied) Characterization of Peroxisome Proliferator-Activated Receptor alpha (PPAR(alpha)) - Independent Effects of PPAR(alpha) Activators in the Rodent Liver: Di-(2-ethylhexyl) phthalate Activates the Constitutive Activated Receptor data files in this series indicate the involvement of PPAR(alpha) and CAR regulatory pathway after DEHP treatment. Keywords: gene expression/microarray

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3748

Platform:

GPL6246

15 Samples

Download data: CEL

Analysis of livers from peroxisome proliferator-activated receptor (PPAR) α-null animals exposed to the plasticizer di-(2-ethylhexyl) phthalate (DEHP). DEHP increases liver tumors in the absence of PPARα. Results provide insight into DEHP-induced transcriptional changes independent of PPARα.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL6246

Series:

GSE18564

15 Samples

Download data: CEL

(Submitter supplied) The nuclear receptor PPARalpha is recognized as the primary target of the fibrate class of hypolipidemic drugs and mediates lipid lowering in part by activating a transcriptional cascade that induces genes involved in the catabolism of lipids. We report here the characterization of three novel PPARalpha agonists with therapeutic potential for treating dyslipidemia. These structurally related compounds display potent and selective binding to human PPARalpha and support robust recruitment of coactivator peptides in vitro. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

30 Samples

Download data: CEL

(Submitter supplied) Acute effects caused by the non-genotoxic carcinogen and peroxisome proliferator (PP) diethylhexylphthalate (DEHP) in the mouse liver

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

24 Samples

Download data: CEL

(Submitter supplied) Fasting, fed, and Clofibric Acid comparisons Keywords: other

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL518

6 Samples

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(Submitter supplied) cDNA microarray from porcine muscle cDNA library

Organism:

Sus scrofa

1 Series

6 Samples

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(Submitter supplied) Approaches for discovering mechanisms of action and for identifying molecular biomarkers in biomedical research are evolving today, as the growing symbiosis with computational sciences becomes more widely appreciated than ever. In fact, the combination of various new technologies has been pushing forward both frontiers. Here, we present an example of the combined use of in vivo siRNA knock-down technology, genome-wide gene expression profiling, and computational reasoning to unveil regulatory causal relationships and the sufficiency network of identified genes for compound-induced toxicity. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4696

20 Samples

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(Submitter supplied) Background: Peripheral blood mononuclear cells (PBMCs) are relatively easily obtainable cells in humans. Gene expression profiles of PBMCs have been shown to reflect the pathological and physiological state of a person. Recently, we showed that the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARα) has a functional role in human PBMCs during fasting. However, the extent of the role of PPARα in human PBMCs remains unclear. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6791

12 Samples

Download data: CEL

(Submitter supplied) Studies in mice have shown that PPARα is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARα in human liver. Here we set out to compare the function of PPARα in mouse and human hepatocytes via analysis of target gene regulation. Primary hepatocytes from 6 human and 6 mouse donors were treated with PPARα agonist Wy14643 and gene expression profiling was performed using Affymetrix GeneChips followed by a systems biology analysis. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1261 GPL570

48 Samples

Download data: CEL

(Submitter supplied) Studies in mice have shown that PPARα is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARα in human liver. Here we set out to compare the function of PPARα in mouse and human hepatocytes via analysis of target gene regulation. Primary hepatocytes from 6 human and 6 mouse donors were treated with PPARα agonist Wy14643 and gene expression profiling was performed using Affymetrix GeneChips followed by a systems biology analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) Studies in mice have shown that PPARα is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARα in human liver. Here we set out to compare the function of PPARα in mouse and human hepatocytes via analysis of target gene regulation. Primary hepatocytes from 6 human and 6 mouse donors were treated with PPARα agonist Wy14643 and gene expression profiling was performed using Affymetrix GeneChips followed by a systems biology analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

24 Samples

Download data: CEL

(Submitter supplied) F344 rats were divided into 4 groups: vehicle control, DEN, DEN+CLO and CLO. After one week of basal diet, rats belonging to the groups DEN and DEN+CLO underwent intraperitoneal injection of DEN (30mg/kg body weight) dissolved in NaCl 9‰. The two other groups were injected with NaCl 9‰ alone. At 30mg/kg, DEN is non-necrogenic thus only exhibiting initiating properties. Twelve days after injection, diet from rats belonging to the groups CLO and DEN+CLO was changed for diet containing 5000ppm CLO for up to 608 days. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL341

176 Samples

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(Submitter supplied) This is an evaluation of the impact of the successive steps of LCM procedure on gene expression profiling by comparing profiles from LCM samples to those obtained with non-microdissected liver samples collected after a one month CLO treatment in the rat. We showed that the H&E staining used for identifying PP-foci and the laser microdissection itself do not impact on RNA quality. Keywords = Microdissection Keywords = Clofibric Acid Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS518

Platform:

GPL85

52 Samples

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Analysis of mechanisms of clofibric acid (CLO) induced hepatocarcinogenesis. Whole liver and early pre-neoplastic foci isolated by laser capture microdissection (LCM) examined.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 5 cell type, 3 dose sets

Platform:

GPL85

Series:

GSE691

52 Samples

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(Submitter supplied) The application of gene expression profiling technology to examine multiple genes and signaling pathways simultaneously promises a significant advance in understanding toxic mechanisms to ultimately aid in protection of public health. Public and private efforts in the new field of toxicogenomics are focused on populating databases with gene expression profiles of compounds where toxicological and pathological endpoints are well characterized. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL219

85 Samples

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(Submitter supplied) This array was developed in-house at NIEHS, and used for gene expression profiling experiments. A complete listing of the genes on this chip is available at the following Web site: http://dir.niehs.nih.gov/microarray/chips.htm cDNA microarray chips were prepared according to DeRisi et al., 1996, Nat. Genet. 14, 457-460. The spotted cDNAs were derived from a collection of sequence-verified clones that covered the 3` end of the gene and ranged in size from 500 to 2000 base pairs (Research Genetics). more...

Organism:

Rattus norvegicus

1 Series

85 Samples

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