GEO DataSets

Expression profiling of AG01518 foreskin fibroblasts at various time points up to 24 hours following stimulation with 10 ng/ml platelet-derived growth factor (PDGF). PDGF is a potent mitogen for fibroblasts and other cells of mesenchymal origin. Results identify genes regulated by PDGF.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 time sets

Platform:

GPL506

Series:

GSE1484

18 Samples

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(Submitter supplied) Subconfluent AG01518 fibroblasts were starved for 16 hours in the presence of 0.1% BSA, and then stimulated for 1, 4, 10 or 24 hours with PDGF-BB (10 ng/ml in starvation medium), or left untreated for the same periods of time. Total RNA was isolated using the RNeasy kit (Qiagen). The RNA quality was checked by formaldehyde agarose gel electrophoresis (RNeasy protocol, Qiagen). Total RNA (40 mg) from cells treated for a given period of time with PDGF or starvation medium alone were labelled in reverse transcription reactions (Superscript II kit, Invitrogen) with dCTP-Cy5 and dCTP-Cy3, respectively (Amersham). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1768

Platform:

GPL506

18 Samples

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(Submitter supplied) Overall study: Identification of PDGF-dependent patterns of gene expression in U87 glioblastoma cells. RNA was obtained from triplicate dishes of 5 different groups of U87 cells, each (total 15) analyzed with one U95 microarray chip. Three different comparisons were made: 1) Clone 3.1 (34580-34582) vs. clone 3.3 (34583-34585) vs. parent U87 (34592-34594). Purpose: demonstrate that the gene expression profiles between these 3 cell lines are not different, so they could be pooled as a single untreated group. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1730

Platform:

GPL8300

15 Samples

Download data: CEL, EXP

Analysis of U87 glioblastoma (GBM) cell clones overexpressing a dominant-negative form of platelet-derived growth factor (PDGF) A subunit to inactive the PDGF autocrine signaling loop. The autocrine PDGF loop is a hallmark of GBM. Results identify genes regulated by oncogenic PDGF signaling.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 genotype/variation sets

Platform:

GPL8300

Series:

GSE1923

15 Samples

Download data: CEL, EXP

(Submitter supplied) Sterol-regulatory element binding proteins (SREBP) control the expression of most genes involved in fatty acid and cholesterol metabolism. In this report, using microarrays, we showed that SREBP1 also induced genes unrelated to lipid biosynthesis, such as heme oxygenase 1 (HMOX1), the phosphatidylinositol-3 kinase regulatory subunit p55-gamma, synaptic vesicle glycoprotein 2A (SV2A) and COTE1 (C1orf2). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL506

3 Samples

Download data: TXT