GEO DataSets

(Submitter supplied) The frequent use of rodent hepatic in vitro systems in pharmacological and toxicological investigations challenges extrapolation of in vitro results to the situation in vivo and interspecies extrapolation from rodents to humans. The toxicogenomics approach may aid in evaluating relevance of these model systems for human risk assessment by direct comparison of toxicant-induced gene expression profiles and infers mechanisms between several systems. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

15 Samples

Download data: TXT

(Submitter supplied) The frequent use of rodent hepatic in vitro systems in pharmacological and toxicological investigations challenges extrapolation of in vitro results to the situation in vivo and interspecies extrapolation from rodents to humans. The toxicogenomics approach may aid in evaluating relevance of these model systems for human risk assessment by direct comparison of toxicant-induced gene expression profiles and infers mechanisms between several systems. more...

Organism:

Rattus norvegicus; Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL887 GPL890

33 Samples

Download data: TXT

(Submitter supplied) Zebrafish embryos have been proposed as an attractive alternative model system for hepatotoxicity testing. In this study we determined gene expression responses after exposure to reference hepatotoxicants and controls to identify biomarkers for hepatotoxicity.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL18378

188 Samples

Download data: CEL

(Submitter supplied) Toxicogenomics (tgx) is used as a tool to identify mechanisms and markers of necrosis in C57BL/6 mice treated by oral gavage using acetaminophen (APAP), isoniazid (INZ), and paraquat (PQ). Critical doses for tgx analysis were derived from a 25 day dose range finding study. For tgx analysis, livers of mice were collected 1 and 2 days after a single compound dose of 168.75, 225, and 300 mg/kg bw for APAP; 12.5, 25, and 50 mg/kg bw for PQ; and 22, 44, and 88 mg/kg bw for INZ. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17226

69 Samples

Download data: CEL

(Submitter supplied) Toxicogenomics (tgx) is used as a tool to identify mechanisms and markers of steatosis in C57BL/6 mice treated by oral gavage using amiodarone (AMD), valproic acid (VPA), and tetracycline (TET). Critical doses for tgx analysis were derived from a 25 day dose range finding study. For tgx analysis, livers of mice were collected after 1, 4, and 11 days of repeated treatment with 6.7, 20, and 60 mg/kg bw for AMD; 125, 250, and 500 mg/kg bw for VPA; and 14.8, 44, and 133 mg/kg bw for TET.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17226

95 Samples

Download data: CEL

(Submitter supplied) Mechanism-based toxicogenomics (tgx) is used as a tool to identify markers reflective of the onset and progression of cholestasis in C57BL/6 mice using Cyclosporin A (CsA) as a model compound. Critical doses for tgx analysis were derived from a dose range finding study in which increase of serum cholesterol, total bile acids, and total bilirubin as well as induction of hepatocyte vacuolization 25 days upon repeated CsA administration through oral gavage were considered as critical effects. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL14178

58 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

300 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with methapyrilene treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (3 uM and 100 uM) of methaphyriline and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with WY-14643 treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses 8 uM and 200 uM) of WY-14643 and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with clofibrate treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (60 uM and 1 mM) of clobibrate and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with diisononyl phthalate treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (25 mM and 100 mM) of diisononyl phthalate and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with chlorpromazine HCl treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (0.8 uM and 20 uM) of chlorpromazine HCl and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with beta-naphthaflavone treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses 1 uM and 100 mM) of beta-naphthaflavone and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with phenobarbital treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (300 uM and 3 mM) of phenobarbital and water vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with sodium valproate treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (500 uM and 10 mM) of sodium valproate and water vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with dioctyl phthalate treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (250 uM and 1 mM) of dioctyl phthalate and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with acetominophen treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (500 uM and 5 mM) of acetominophen and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

30 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptomics is developing into an invaluable tool in toxicology. The aim of this study was, using a transcriptomics approach, to identify genes that respond similarly to chemicals (including drugs and industrial compounds) in both rat liver in vivo and in cultivated hepatocytes, which are up- or downregulated by many different test compounds. For this purpose, we analyzed Affymetrix gene array data from 162 compounds that were previously tested in a concentration-dependent manner in rat livers in vivo and rat hepatocytes cultivated in sandwich culture. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

547 Samples

Download data: CEL, XLSX

(Submitter supplied) At present, substantial efforts are focused on the development of in vitro assays coupled with ”omics” technologies for the identification of carcinogenic substances as an alternative to the classical 2-year rodent carcinogenicity bioassay. A prerequisite for the eventual regulatory acceptance of such assays, however, is the in vivo relevance of the observed in vitro findings.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

72 Samples

Download data: CEL

(Submitter supplied) It is considered that read-across based on only structure similarity has a risk of error on risk assessment. Under this circumstances, considering biological similarity based on adverse outcome pathways (AOPs) using in vitro omics technologies is expected to enhance the accuracy and robustness of conclusions in read-across. However, because of a lack of practical case studies, it is not well discussed key considerations and how to use these technologies for data gap filling. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL22740

18 Samples

Download data: TXT