GEO DataSets

(Submitter supplied) Background: Our understanding of the role host genetic factors play in the initiation and severity of infections caused by Gram negative bacteria is incomplete. Methods: To identify novel regulators of the host response to lipopolysaccharide (LPS), 11 inbred murine strains were challenged with LPS systemically. RNA from lung, liver, and spleen tissue was profiled on oligonucleotide microarrays to determine if unique transcripts differentiate susceptible and resistant strains of mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6466

264 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

synthetic construct; Mus musculus

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL7202 GPL8786

64 Samples

Download data: CEL, TXT

(Submitter supplied) Our recent study of gene expression in mice treated with LPS systemically identified the E2F1 transcription factor as a novel regulator of innate immune response in lung, liver, and spleen tissue. Our follow up studies showed that RNAi-mediated inhibition or E2F1 gene deficiency lead to reduced inflammatory response to LPS in vitro and in vivo. Furthermore, a clear role for the role of miRNAs in the regulation of innate immune response to LPS has emerged. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

32 Samples

Download data: TXT

(Submitter supplied) Our recent study of gene expression in mice treated with LPS systemically identified the E2F1 transcription factor as a novel regulator of innate immune response in lung, liver, and spleen tissue. Our follow up studies showed that RNAi-mediated inhibition or E2F1 gene deficiency lead to reduced inflammatory response to LPS in vitro and in vivo. Furthermore, a clear role for the role of miRNAs in the regulation of innate immune response to LPS has emerged. more...

Organism:

Mus musculus; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL8786

32 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL256

15 Samples

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(Submitter supplied) Temporal analysis of bone marrow derived macrophages after 10 ug/ml lipopolysaccharide stimulation. C57BL6, C3H/ARC, BalbC and C3H/HeJ mouse strains analyzed. Keywords: time course

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL256

18 Samples

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(Submitter supplied) Paeony root has long been used for its anti-inflammatory effects. In this study, the effects of albiflorin, paeoniflorin, and paeonol, compounds from paeony root, on gene expression profiles were examined in macrophages. The RAW264.7 macrophages were treated with LPS in the presence or absence of albiflorin, paeoniflorin, or paeonol. Global mRNA expression levels were detected by using an oligonucleotide microarray platform covering the mouse whole genome. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

33 Samples

Download data: TXT

(Submitter supplied) These microarrays were performed for use in a genome-wide scan for LPS-regulated genes in mouse macrophages, in order to construct a list of LPS-regulated genes for detailed interrogation on custom microarrays (see GSE19490 for custom array analysis).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4055

11 Samples

Download data: TXT

(Submitter supplied) These microarrays were performed for use in a genome-wide scan for LPS-regulated genes in human monocyte-derived macrophages, in order to construct a list of LPS-regulated genes for detailed interrogation on custom microarrays (see GSE19482 for custom array analysis).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

10 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

4 related Platforms

61 Samples

Download data: TXT

(Submitter supplied) Evolutionary change in gene expression is generally considered to be a major driver of phenotypic differences between species. We investigated innate immune diversification by analyzing inter-species differences in the transcriptional responses of primary human and mouse macrophages to the TLR4 agonist, LPS. Using a custom platform permitting cross-species interrogation coupled with deep sequencing of mRNA 5’ ends, we identified extensive divergence in LPS-regulated orthologous gene expression between humans and mice (24% of orthologs, http://www.macgate.qfab.org). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL9802

24 Samples

Download data: TXT

(Submitter supplied) Evolutionary change in gene expression is generally considered to be a major driver of phenotypic differences between species. We investigated innate immune diversification by analyzing inter-species differences in the transcriptional responses of primary human and mouse macrophages to the TLR4 agonist, LPS. Using a custom platform permitting cross-species interrogation coupled with deep sequencing of mRNA 5’ ends, we identified extensive divergence in LPS-regulated orthologous gene expression between humans and mice (24% of orthologs, http://www.macgate.qfab.org). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9801

16 Samples

Download data: TXT

(Submitter supplied) 60-mer spotted oligonucleotide probes. Protocol: See manufacturer's website (www.agilent.com).

Organism:

Mus musculus

2 Series

24 Samples

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(Submitter supplied) 60-mer spotted oligonucleotide probes. Protocol: See manufacturer's website (www.agilent.com).

Organism:

Homo sapiens

2 Series

16 Samples

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(Submitter supplied) In airway epithelial cells, Tlr4 mediates both innate and adaptive immune responses to viral pathogens through the surface fusion glycoprotein (RSV F), however the mechanism is not well characterized. We used microarrays to identify differential gene expression pathways induced by RSV infection.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

44 Samples

Download data: CEL, CHP

(Submitter supplied) In airway epithelial cells, Tlr4 mediates both innate and adaptive immune responses to viral pathogens through the surface fusion glycoprotein (RSV F), however the mechanism is not well characterized. We used microarrays to identify Tlr4-mediated gene expression pathways induced by RSV infection.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

18 Samples

Download data: CEL, CHP

(Submitter supplied) Microarray analysis of whole brain RNA collected 48 hr after LPS (4mg/kg i.p.) injection We induced systemic inflammation by injecting mice with LPS. We then performed global gene expression profiling of whole brain RNA and looked for genes that may be involved in behavioural changes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) Gene-expression microarray datasets generated as part of the Immunological Genome Project (ImmGen). Primary cells from multiple immune lineages are isolated ex-vivo, primarily from young adult B6 male mice, and double-sorted to >99% purity. RNA is extracted from cells in a centralized manner, amplified and hybridized to Affymetrix 1.0 ST MuGene arrays. Protocols are rigorously standardized for all sorting and RNA preparation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

653 Samples

Download data: CEL

(Submitter supplied) Salmonella causes inflammation in infected hosts. Inflammation is a well-characterized defensive mechanism of innate immunity. The recognition and engagement of lipopolysaccharide (LPS) endotoxins in the outer membranes of Salmonella to Toll-like receptor 4 of immune cells (macrophages and dendritic cells) trigger inflammatory responses characterized by secretion of pro-inflammatory cytokines, including TNF-beta, IL-1 and IL-6. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL13457

68 Samples

Download data: XLS

(Submitter supplied) Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL256

5 Samples

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