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Links from GEO DataSets

Items: 9

1.

Digital transcriptome analysis of genes on sex chromosomes in papaya (Carica papaya)

(Submitter supplied) For identifying genes for sex determination in papaya, digital gene expression analysis by Ht-SuperSAGE (Matsumura et al., 2010) was carried out in flowers from male, female and hermaphrodite plants of papaya. Total more than 9,273,744 26bp-tags were obtained by sequence analysis using SOLiD3 and mapped on papaya primitive sex chromosome sequences.
Organism:
Carica papaya
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13809
6 Samples
Download data
Series
Accession:
GSE30366
ID:
200030366
2.

Small RNA Solexa sequencing of multiple tissues of papaya, Carica papaya

(Submitter supplied) The tissues of male flowers, female flowers and hermaphrodite are collected and stored in liquid nitrigen immediately. Total RNA was isolated from male, hermaphrodite, and female flowers using the Trizol reagent (Invitrogen USA). Low molecular weight RNA was enriched from the total RNA by precipitating with 0.4M NaCl and polyethylene glycol (PEG). The enriched low molecular weight RNA was then separated on a denaturing 15% polyacrylamide gel. more...
Organism:
Carica papaya
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL13438
6 Samples
Download data: TXT
Series
Accession:
GSE54097
ID:
200054097
3.

Differential gene expression among three sex types reveals a MALE STERILITY 1 (CpMS1) for sex differentiation in papaya

(Submitter supplied) The aim of this study was to identify differentially expressed genes among male, female and hermaphrodite flowers in papaya during early (pre-meiosis) and later (post-meiosis) stages of flower development. We identified a Male Sterility 1 gene (CpMS1) highly up-regulated in male and hermaphrodite flower buds compared to female flower buds. Besides important transcription factors related to flower organ development and flowering time regulation, we identified differential expression of genes that are known to participate in ABA, ROS and auxin signaling pathways.
Organism:
Carica papaya
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL27469 GPL26326
12 Samples
Download data: FA, TXT
Series
Accession:
GSE137547
ID:
200137547
4.

Characterization and analysis of the promoter region of Monodehydroascorbate reductase 4 (CpMDAR4) in papaya

(Submitter supplied) The aim of this research was to study the effects of cis-element differences between the X, Y and Yh alleles on the expression of CpMDAR4, a potential candidate gene for sex differentiation in papaya, using a transcriptional reporter system in a model species Arabidopsis thaliana. Possible effects of a retrotransposon insertion in the Y and Yh alleles on the transcription and expression of CpMDAR4 alleles in papaya flowers were also examined. more...
Organism:
Carica papaya
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26326
55 Samples
Download data: TXT
Series
Accession:
GSE208265
ID:
200208265
5.

Plant Y-chromosome degeneration is retarded by haploid purifying selection.

(Submitter supplied) Sex chromosomes evolved many times independently in many different organisms. According to the currently accepted model, X- and Y-chromosomes evolve from a pair of autosomes via a series of inversions leading to step-wise expansion of a non-recombining region on the Y chromosome (NRY) and the consequential degeneration of genes trapped in the NRY. Our results suggest that plants represent an exception to this rule due to their unique life-cycle that includes alteration of diploid and haploid generations and widespread haploid expression of genes in plant gametophytes. more...
Organism:
Silene latifolia; Silene vulgaris
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL13973 GPL13974
11 Samples
Download data
Series
Accession:
GSE30958
ID:
200030958
6.

Rapid de novo evolution of X chromosome dosage compensation in Silene latifolia, a plant with young sex chromosomes

(Submitter supplied) Silene latifolia is a dioecious plant with heteromorphic sex chromosomes that have originated only ~10 MYA and is a promising model organism to study sex chromosome evolution in plants. Previous work suggests that S. latifolia XY chromosomes have gradually stopped recombining and the Y chromosome is undergoing degeneration as in animal sex chromosomes. However, this work has been limited by the paucity of sex-linked genes available. more...
Organism:
Silene latifolia
Type:
Expression profiling by high throughput sequencing
Platform:
GPL15196
6 Samples
Download data
Series
Accession:
GSE35563
ID:
200035563
7.

Deep sequencing and analysis of small RNAs from the flowers of four Rosa

(Submitter supplied) High-throughput sequencing was employed to identify conserved and variable microRNAs among 4 Rosa
Organism:
Rosa rugosa; Rosa hybrid cultivar
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL15890 GPL15889
4 Samples
Download data: TXT
Series
Accession:
GSE39882
ID:
200039882
8.

Effects of proto-Y chromososomes and temperature on house fly gene expression

(Submitter supplied) Purpose: House fly has a stable polygenic sex determination system. The male determining factor (Mdmd) is commonly found on the Y chromosome (Y^M) or the third chromosome (III^M). These proto-Y chromosomes are clinally distributed, with Y^M found most commonly in northern latitudes and III^M most commonly found at southern latitudes, hinting at possible genotype-by-temperature interactions that maintain the polymorphism. more...
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL22576
24 Samples
Download data: CSV
Series
Accession:
GSE136188
ID:
200136188
9.

Identification and validation of key genes related to preferred flavour profiles in Australian commercial papaya (Carica papaya L.)

(Submitter supplied) To uncover a suit of genes related to the consumer preferred flavours, whole RNA sequencing followed by de novo genome assembly was performed on extreme flavoured papaya varieties RB1 (preferred with sweet flavour and floral aroma) and 1B (non-preferred with bitter flavour and musty aroma) fruits at ripe and unripe stages. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different papaya varieties at ripe and unripe stages.
Organism:
Carica papaya
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32671
12 Samples
Download data: FASTA, TXT
Series
Accession:
GSE213436
ID:
200213436
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