GEO DataSets

(Submitter supplied) Analysis of gene expression levels in ex-vivo and p79-stimulated splenic CD4+ T cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL14828

12 Samples

Download data: TXT

(Submitter supplied) Type 1 Diabetes (T1D) in humans and the non-obese diabetic (NOD) mouse model results from autoreactive T cell destruction of pancreatic beta cells. A pathogenic role for B lymphocytes (B cells) in T1D first became evident when NOD mice made deficient in this population through introduction of an inactivated Igµ heavy chain gene (NOD.Igµnull) or chronic treatment with anti-IgM antibodies were strongly protected from disease. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4340

Platform:

GPL1261

11 Samples

Download data: CEL

Analysis of anti-IgM-F(ab’)2 fragment-stimulated, splenic B cells from non-obese diabetic (NOD) or NR4 (NOD background with Chromosome 4, type 1 diabetes (T1D)-resistance alleles) females. Results provide insight into the molecular mechanisms underlying NOD and NR4 diabetogenic activity.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 2 strain sets

Platform:

GPL1261

Series:

GSE37294

11 Samples

Download data: CEL

(Submitter supplied) We used high density oligonucleotide arrays to measure the relative expression levels of >39,000 genes and ESTs in the NOD mouse (a murine model of T1D and other autoimmune conditions), four NOD-derived diabetes resistant congenic strains and two nondiabetic control strains. Owing to the importance of T cells in the development of T1D we decided to target two organs, the spleen and thymus. We profiled gene expression in thymi from four week old female mice and spleens from three month old female mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS10

Platform:

GPL24

28 Samples

Download data

(Submitter supplied) Custom Affymetrix GeneChip designed by Eos Biotechnology Inc. to measure the expression of a large number of genes or ESTs on a single array. This is achieved by choosing those probes from within each set of 20 Affymetrix perfect match probes that show the least random fluctuation relative to the perceived specific hybridization over a wide ranging set of samples (Glynne et al. 2000). Experiments undertaken by Eos have revealed that the mismatch probes do not increase sensitivity or reproducibility (RJG and GG unpublished data). more...

Organism:

Mus musculus

1 DataSet

3 Series

28 Samples

Download data

Examination of spleen and thymus of type 1 diabetes nonobese diabetic (NOD) mouse, four NOD-derived diabetes-resistant congenic strains and two nondiabetic control strains.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 disease state, 7 strain, 2 tissue sets

Platform:

GPL24

Series:

GSE11

28 Samples

Download data

(Submitter supplied) We demonstrate diverse roles of interferon–gamma (IFN-γ) in the induction and regulation of immune-mediated inflammation using a transfer model of autoimmune diabetes. The diabetogenic CD4+BDC2.5 (BDC) T cell clone upon transfer into NOD.scid mice induced destruction of islets of Langerhans leading to diabetes. Administration of a neutralizing antibody to IFN-γ (H22) resulted in long term protection (LTP) from diabetes, with inflammation but persistence of a significant, albeit decreased numbers of β-cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4034

Platform:

GPL1261

8 Samples

Download data: CEL

Temporal analysis of microdissected islets of NOD.Scid mice injected with diabetogenic CD4+BDC2.5 (BDC) T cells, then injected with anti-interferon-γ monoclonal antibody which protected the mice from developing diabetes. Results provide insight into the role of INF-γ role in autoimmune diabetes.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 2 protocol, 5 time sets

Platform:

GPL1261

Series:

GSE12389

8 Samples

Download data: CEL

(Submitter supplied) Type 1 diabetes is a multigenic disease caused by T-cell mediated destruction of the insulin producing β-cells. Although conventional (targeted) approaches of identifying causative genes have advanced our knowledge of this disease, many questions remain unanswered. Using a whole molecular systems study, we unraveled the genes/molecular pathways that are altered in CD4 T-cells from young NOD mice prior to insulitis (lymphocytic infiltration into the pancreas). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS5018 GDS5019 GDS5020

Platform:

GPL1261

44 Samples

Download data: CEL

Analysis of CD4 T-cells from 4-week old NOD females in the preinsulitis stage of Type 1 diabetes. Control strains NOR (~88% similarity to NOD genome) and C57BL/6 (genetically distant) are both insulitis- and diabetes-free. Results provide insight into molecular basis of CD4 T-cell diabetogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state, 3 strain sets

Platform:

GPL1261

Series:

GSE46600

15 Samples

Download data: CEL

Analysis of CD4 T-cells from 3-week old NOD females in the preinsulitis stage of Type 1 diabetes. Control strains NOR (~88% similarity to NOD genome) and C57BL/6 (genetically distant) are both insulitis- and diabetes-free. Results provide insight into molecular basis of CD4 T-cell diabetogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state, 3 strain sets

Platform:

GPL1261

Series:

GSE46600

15 Samples

Download data: CEL

Analysis of CD4 T-cells from 2-week old NOD females in the preinsulitis stage of Type 1 diabetes. Control strains NOR (~88% similarity to NOD genome) and C57BL/6 (genetically distant) are both insulitis- and diabetes-free. Results provide insight into molecular basis of CD4 T-cell diabetogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state, 3 strain sets

Platform:

GPL1261

Series:

GSE46600

14 Samples

Download data: CEL

(Submitter supplied) In an accompanying paper we found specific localization of diabetogenic T cells only to islets of Langerhans bearing the specific antigen. Instrumental in the specific localization was the presence of intra-islet dendritic cells bearing the β-cell-peptide-MHC complex. Here we report that the entry of diabetogenic CD4 T cells very rapidly triggered inflammatory gene expression changes in islets and vessels by up-regulating chemokines and adhesion molecules. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4115 GDS4116

Platform:

GPL6246

20 Samples

Download data: CEL, CHP

Analysis of pancreatic islets after injection of diabetogenic BDC CD4 T cells into nondiabetic NOD Rag1-/- recipients, 24 to 48 hrs post-transfer. Results provide insight into molecular mechanisms underlying the T-cell entry induced state of receptivity of islets to subsequent immunological insults.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL6246

Series:

GSE26147

13 Samples

Download data: CEL, CHP

Analysis of islet cells after injection of diabetogenic 3A9 CD4 T cells into nondiabetic B10.BR IP-HEL recipients, 8 to 24 hrs post-transfer. Results provide insight into molecular mechanisms underlying the T-cell entry induced state of receptivity of islets to subsequent immunological insults.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol, 3 time sets

Platform:

GPL6246

Series:

GSE26147

7 Samples

Download data: CEL, CHP

(Submitter supplied) The aim of this study is to identify genes implicated in the early steps of the autoimmune process, prior to inflammation in type 1 diabetes. Early Insulin AutoAntibodies (E-IAA) have been used as subphenotypic marker to select individual animals as type 1 diabetes prone and to compare gene expression patterns with insulin autoantibody negative NOD. Variation of gene expression patterns in the pancreatic lymph nodes (PLN) issued from E-IAA positive and negative mice have been analyzed by DNA microarrays

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4024

Platform:

GPL81

9 Samples

Download data: CEL

Analysis of pancreatic lymph nodes of Early Insulin AutoAntibodies (E-IAA) positive 5 wk-old Non Obese Diabetic (NOD) mice. E-IAA mark the first measurable phenotypic checkpoint in T1D pathogenesis. Results provide insight into molecular mechanisms underlying initiation of T1D.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL81

Series:

GSE15582

9 Samples

Download data: CEL

(Submitter supplied) To gain further insight into potential specific gene signatures expressed by pancreatic islet cells and pathogenic CD4+ T lymphocytes isolated from sublines of non-obese diabetic (NOD) mice expressing high or low autoimmune (type 1) diabetes incidence.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

50 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by RT-PCR; Expression profiling by high throughput sequencing

Platforms:

GPL26927 GPL16417

1845 Samples

Download data: XLS, XLSX

(Submitter supplied) Abstract- The class II region of the Major Histocompatibility locus is the main contributor to the genetic susceptibility to type 1 diabetes (T1D). The loss of an aspartic acid at position 57 of diabetogenic HLA-DQ8 chains supports this association; it influences the recognition of peptides in the context of HLA-DQ8, and I-Ag7 using a mechanism termed the P9 switch. Here, we built register-specific insulin MHC tetramers, Ins12-20 and Ins13-21, to examine anti-insulin CD4 T cell responses during the early pre-diabetic phase of disease in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16417

16 Samples

Download data: XLS, XLSX