GEO DataSets

(Submitter supplied) We report the miRNA profile of extracellular vesicles released from Atlantic salmon head kidney white blood cells that have been cultured for 1 day or 5 days.

Organism:

Salmo salar

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL27980

8 Samples

Download data: CSV

(Submitter supplied) Transcriptional profiling of Atlantic salmon adherent HKLs that were cultured for 1 day (24 h) and cultured for 5 days (120 h). Our previous data (RNA-seq, morphology and phagocytosis) indicate that Day 1 cells are predominatly monocytes while Day 5 cells are predominatly macrophages.

Organism:

Salmo salar

Type:

Expression profiling by array

Platform:

GPL11299

8 Samples

Download data: TXT

(Submitter supplied) Exosomal RNA isolated from plasma was successfully profiled with a total of 65 immunology-related miNRAs in 13 mouse samples

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL25310

13 Samples

Download data: TXT

(Submitter supplied) In this study, the methods to isolate and identify extracellular vesicles (EVs) including exosomes, from the seminal plasma (SP) of 3 fertile (F) and subfertile (S) bucks have been developed. Additionally, we investigated whether specific miRNA abundance differences between F and SF bucks could serve as fertility biomarkers. Ultracentrifugation and size exclusion chromatography analysis have made it possible to isolate different SP-EVs concentrations (8.53x10^11 ± 1.04x10^11 and 1.84x10^12 ± 1.75x10^11 particles/ml of SP; p=0,008), with a similar average size (143.9 ± 11.9 and 115.5 ± 2.4 nm; p=0.7422) in F and S males, respectively. more...

Organism:

Oryctolagus cuniculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26786

6 Samples

Download data: XLSX

(Submitter supplied) The role of extracellular vesicles (EVs), specifically exosomes, in intercellular communication likely plays a key role in placental orchestration of pregnancy and maternal immune sensing of the fetus. While murine models are powerful tools to study pregnancy and maternal-fetal immune interactions, in contrast to human placental exosomes, the content of murine placental and pregnancy exosomes remains largely understudied. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16417

12 Samples

Download data: TSV, TXT

(Submitter supplied) The goal of this study is to determine if infection with flavivirus (West Nile virus) and action of proinflamatory cytokine interferon alter incorporation of host RNAs into extracellular vesicles secreted by human cells.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: XLS

(Submitter supplied) Moritella viscosa is a bacterial pathogen causing winter-ulcer disease in Atlantic salmon. The lesions on affected fish lead to increased mortality, decreased fish welfare, and inferior meat quality in farmed salmon. MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional regulation by guiding the miRNA induced silencing complex to specific mRNA transcripts (target genes). The goal of this study was to identify miRNAs responding to Moritella viscosa in salmon by investigating miRNA expression in head-kidney and the muscle/skin from lesion sites caused by the pathogen. more...

Organism:

Salmo salar

Type:

Expression profiling by array

Platform:

GPL28080

23 Samples

Download data: TXT

(Submitter supplied) We aim to explore the transcriptome of the dermis of the bleomycin induced murine scleroderma model which were treated with PBS or bone marrow stem cell derived exosome.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: XLSX

(Submitter supplied) We aim to explore the types and expression of miRNA in the mouse bone marrow stem cell derived exosome.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17021

2 Samples

Download data: XLSX

(Submitter supplied) RNA was harvested from 4T1 tumors in mice treated with miR-loaded EVs

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

12 Samples

Download data: TXT

(Submitter supplied) RNA was harvested from RAW 264.7 cells treated with miR-loaded extracellular vesicles

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

12 Samples

Download data: TXT

(Submitter supplied) RNA was harvested from endothelial cells cultured alone or with tumor cells and the associated endothelial extracellular vesicles.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: CSV

(Submitter supplied) We performed RNA-seq on human iPSC derived astrocytes and iPSC derived astrocyte derived extracellular vesicles.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24676

4 Samples

Download data: TAB

(Submitter supplied) Extracellular vesicles (EVs) and their microRNA (miRNA) cargo have been suggested as potential biomarkers for mammary gland health in cattle. However, milk is a dynamic fluid, and its biologically active components, including miRNAs, could be subject to changes throughout the day. The current study aimed to evaluate the circadian fluctuation of milk EVs miRNA cargo to assess the feasibility of milk EVs as future biomarkers for mammary gland health management. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19172

28 Samples

Download data: XLSX

(Submitter supplied) A study was conducted to determine if different levels of vegetable and fish oils can alter antiviral responses of salmon macrophage-like cells (MLCs). Atlantic salmon were fed diets containing 7.38% (FO7) or 5.09% (FO5) fish oil. These diets were designed to be relatively low in EPA+DHA (i.e. FO7: 1.41% and FO5: 1%), but near the requirement level, resulting in no significant change in salmon growth. more...

Organism:

Salmo salar

Type:

Expression profiling by array

Platform:

GPL11299

24 Samples

Download data: TXT

(Submitter supplied) Head kidney (HK) and distal intestine (DI) from Atlantic salmon (average 550g) were collected. Immune cells from DI and HK were extracted and grown at 12°C in Leibovitz’s L-15 Medium (L-15). The isolated DI or HK leukocytes were allowed to adhere on a petri dish with 2 mL L-15+ for 2 days at 12°C. After removing the supernatant containing non-adherent cells, the adherent cells on petri dish were detached by washing three times with 1.5 mL ice-cold PBS supplemented with 5 mM EDTA. more...

Organism:

Salmo salar

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28848

12 Samples

Download data: TXT

(Submitter supplied) Atlantic salmon (average 550g) were used in this study. The fish were starved for 24 h and were sacrificed with an overdose of MS-222. Then head kidney (HK) and distal intestine (DI) samples were collected. Immune cells from HK and DI were isolated and cultured at 12°C in Leibovitz’s L-15 Medium (L-15). Both HK and DI leukocytes were allowed to adhere on a petri dish with 2 mL L-15+ for 2 days at 12°C. more...

Organism:

Salmo salar

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28848

12 Samples

Download data: TXT

(Submitter supplied) Purpose: Macrophages are often classified into M1 ‘classical’ and M2 ‘alternatively-activated’ macrophages. Extracellular vesicles (EVs) are biomolecule carriers involved in cell-cell communication. Here, we provide a first insight into the complete small RNA cargo of human macrophage M1/M2 EVs. Methods: Monocyte-derived macrophages were polarised into M1 (GM-CSF+LPS+IFNγ) or M2 (M-CSF+IL-4+IL-13) and EVs isolated by size exclusion chromatography. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

12 Samples

Download data: TXT

(Submitter supplied) The goal of this study is to identify unique miRNA profiles of EVs from MCF7 and MCF10A cells that distinguish their cellular origin.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18691

10 Samples

Download data: RCC

(Submitter supplied) MicroRNAs (miRNAs) are a class of small RNA molecules that regulate expression of specific mRNA targets. They can be released from cells, often encapsulated within extracellular vesicles (EVs), and therefore have the potential to mediate intercellular communication. It has been suggested that certain miRNAs may be selectively exported, although the mechanism has yet to be identified. Manipulation of the miRNA content of EVs will be important for future therapeutic applications. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9115

4 Samples

Download data: TXT