GEO DataSets

(Submitter supplied) We found that single-cell RNA-seq (scRNA-seq)dataneeded to have2,000or more cellsin a cluster to identify the majority of differentially expressed genes (DEGs) with modest differences, while as few as 50-100cells weresufficient for identifying the majority of DEGs withextremely small p values or high abundance.The findings provide a quantitativereference for designing and interpreting studies that usescRNA-seq datato identify DEGs.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

12 Samples

Download data: CSV, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Salmonella enterica subsp. enterica serovar Typhimurium

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30637

396 Samples

Download data

(Submitter supplied) In this work, we have improved our previously published bacterial single-cell RNA-sequencing protocol (MATQ-seq), providing enhancements that achieve a higher cell throughput while also including integration of automation. We selected a more efficient reverse transcriptase which led to a lower drop-out rate and higher workflow robustness, and we also successfully implemented a Cas9-based ribosomal RNA depletion protocol into the MATQ-seq workflow. more...

Organism:

Salmonella enterica subsp. enterica serovar Typhimurium

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30637

384 Samples

Download data: TSV

(Submitter supplied) In this work, we have improved our previously published bacterial single-cell RNA-sequencing protocol (MATQ-seq), providing enhancements that achieve a higher cell throughput while also including integration of automation. We selected a more efficient reverse transcriptase which led to a lower drop-out rate and higher workflow robustness, and we also successfully implemented a Cas9-based ribosomal RNA depletion protocol into the MATQ-seq workflow. more...

Organism:

Salmonella enterica subsp. enterica serovar Typhimurium

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30637

12 Samples

Download data: TSV

(Submitter supplied) The goal of this study is to discover fibroblast subpopulations relevant to recessive dystrophic epidermolysis bullosa

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15520

543 Samples

Download data: TXT

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Homo sapiens; Canis lupus familiaris; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26863

2 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Mus musculus; Homo sapiens; Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26865

1 Sample

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Homo sapiens; Canis lupus familiaris; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26866

16 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Mus musculus; Homo sapiens; Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26866

2 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Homo sapiens; Canis lupus familiaris; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24142

1 Sample

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Canis lupus familiaris; Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26867

8 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Canis lupus familiaris; Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26866

4 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Canis lupus familiaris; Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24142

12 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Canis lupus familiaris; Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26865

4 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Canis lupus familiaris; Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24142

6 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Mus musculus; Homo sapiens; Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26864

15 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Homo sapiens; Canis lupus familiaris; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26863

160 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Mus musculus; Homo sapiens; Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26863

140 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Mus musculus; Homo sapiens; Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26863

8 Samples

Download data: TSV

(Submitter supplied) Single-cell RNA sequencing (scRNA-seq) is the leading technique for charting the molecular properties of individual cells. The latest methods are scalable to thousands of cells, enabling in- depth characterization of sample composition without prior knowledge. However, there are important differences between scRNA-seq techniques, and it remains unclear which are the most suitable protocols for drawing cell atlases of tissues, organs and organisms. more...

Organism:

Homo sapiens; Mus musculus; Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26863

2 Samples

Download data: TSV