GEO DataSets

(Submitter supplied) African swine fever (ASF) is a deadly disease of swine currently causing a worldwide pandemic leading to severe economic consequences for the porcine industry. The control of disease spread is hampered by the limitation of available effective vaccines. Live attenuated vaccines (LAVs) are currently the most advanced vaccine prototypes, providing strong protection against ASF. However, the significant advances achieved using LAVs must be complemented with further studies to analyse vaccine-induced immunity. more...

Organism:

Sus scrofa domesticus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29562

60 Samples

Download data: TXT

(Submitter supplied) We describe the transcriptomic recall response to African swine fever virus (ASFV) upon in vitro stimulation in porcine peripheral blood mononuclear cells (PBMC) obtained three weeks after receiving an intranasal dose of 106 pfu of the live attenuated BA71ΔCD2 vaccine prototype. PBMC from 4 unvaccinated and 4 vaccinated pigs were stimulated with BA71ΔCD2 or left untreated for 10 hours, RNA was extracted, and bulk RNA-sequencing was performed. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

16 Samples

Download data: TSV

(Submitter supplied) We describe the recall response to African swine fever virus (ASFV) by applying single-cell RNA-sequencing (scRNA-seq) of submandibular lymph node (LN) cells from a control and a vaccinated animal with the live attenuated BA71ΔCD2 vaccine prototype. Cell suspensions obtained from the digested tissues were in vitro stimulated for 16 hours with BA71ΔCD2 ASFV, and methanol-fixed prior sequencing using the 10x Genomics scRNA-seq platform. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

2 Samples

Download data: H5