GEO DataSets

Analysis of bacterial infected C. elegans treated with 70 μM RPW-24 or DMSO. In a C.elegans-bacterial infection assay screening small molecules, RPW-24 was identified as a putative antimicrobial agent. Results provide insight into C. elegans conserved immune response to bacterial infection.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL200

Series:

GSE37266

6 Samples

Download data: CEL

(Submitter supplied) The nematode Caenorhabditis elegans offers currently untapped potential for carrying out high-throughput, live-animal screens of low molecular weight compound libraries to identify molecules that target a variety of cellular processes. We previously used a bacterial infection assay in C. elegans to identify 119 compounds that affect host-microbe interactions among 37,214 tested. We subsequently found that one of these small molecules, RPW-24, protects C. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS4243

Platform:

GPL200

6 Samples

Download data: CEL

(Submitter supplied) Traditional treatments for bacterial infection have focused upon directly inhibiting growth of the pathogen. However, an equally important determinant of infection outcome is the host defense response. We previously performed a high-throughput chemical screen to identify small molecules that rescued the nematode Caenorhabditis elegans from infection by Pseudomonas aeruginosa. Over 20 of the hits stimulated host defense gene expression. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

18 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL19757 GPL13657

31 Samples

Download data: TDF, WIG

(Submitter supplied) ChIP-seq of ATF-7::GFP from Wild Type or pmk-1 mutant animals exposed to either non-pathogenic E. coli OP50 or pathogenic P. aeruginosa PA14

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13657

14 Samples

Download data: NARROWPEAK, TDF, WIG

(Submitter supplied) RNA-seq of Wild Type (N2), pmk-1 or atf-7 mutant animals exposed to either non-pathogenic E. coli OP50 or pathogenic P. aeruginosa PA14

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19757

17 Samples

Download data: TXT

(Submitter supplied) Synchronized C. elegans cultures of three geontypes -- wildype N2, daf-2(e1370) and sma-6(wk7) -- were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated at 25C for 4 or 24 hours before harvesting. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

4 related Platforms

26 Samples

Download data: TXT

(Submitter supplied) Many pathogens secrete toxins that target key host processes resulting in the activation of immune pathways. The secreted Pseudomonas aeruginosa toxin Exotoxin A (ToxA) disrupts intestinal protein synthesis which triggers the induction of a subset of P. aeruginosa-response genes in the nematode Caenorhabditis elegans. We found that losing one ToxA-induced C. elegans gene, the Tribbles pseudokinase ortholog nipi-3, results in hypersusceptibility to both P. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

9 Samples

Download data: CEL

(Submitter supplied) Very little is known about how animals discriminate pathogens from innocuous microbes. To address this question, we examined infection-response gene induction in the nematode Caenorhabditis elegans. We focused on genes that are induced in C. elegans by infection with the bacterial pathogen Pseudomonas aeruginosa, but are not induced by an isogenic attenuated gacA mutant. Most of these genes are induced independently of known immunity pathways. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

12 Samples

Download data: CEL, TXT

(Submitter supplied) Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark).

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9458

14 Samples

Download data: GPR

(Submitter supplied) Optimal health requires perpetual transcriptional fidelity of gene expression. SKN-1/NRF2 is a cytoprotective transcription factor in C. elegans that regulates the expression of cellular defenses during stress, including: nutrient deprivation, redox imbalance, and xenobiotic and pathogen exposure. Constitutive activation of SKN-1 results in pleiotropic outcomes, including shortened lifespan and protective redistribution of somatic fat to the germline. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

15 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below. Keywords: Expression profiling by high throughput sequencing

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25145

20 Samples

Download data: TXT

(Submitter supplied) Germline-encoded pattern recognition receptors (e.g. Toll-like receptors) play key roles in innate immune activation. However, some metazoans, such as C. elegans, do not have canonical mechanisms of pattern recognition, yet they are able to mount anti-pathogen immune defenses. Here, we demonstrate that a nuclear hormone receptor (NHR), a ligand-gated transcription factor, functions in immune activation and pathogen defense. more...

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25145

2 Samples

Download data: TXT, XLSX

(Submitter supplied) Germline-encoded pattern recognition receptors (e.g. Toll-like receptors) play key roles in innate immune activation. However, some metazoans, such as C. elegans, do not have canonical mechanisms of pattern recognition, yet they are able to mount anti-pathogen immune defenses. Here, we demonstrate that a nuclear hormone receptor (NHR), a ligand-gated transcription factor, functions in immune activation and pathogen defense. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25145

18 Samples

Download data: TXT

(Submitter supplied) Olfactory neurons allow animals to discriminate nutritious food sources from potential pathogens. From a forward genetic screen, we uncovered a surprising requirement for the olfactory neuron gene olrn-1 in the regulation of intestinal epithelial immunity in Caenorhabditis elegans. During nematode development, olrn-1 is required to program the expression of odorant receptors in the AWC olfactory neuron pair. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25145

7 Samples

Download data: XLSX

(Submitter supplied) Aging has been shown to be under genetic control in C. elegans. We performed Affymetrix micorarray-based transcriptional profililng of wild type C. elegans strain Bristol N2 during aging to detect temporal changes in gene expression.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3943

Platform:

GPL200

9 Samples

Download data: CEL

Analysis of C.elegans at 3 points during aging: late larval L4 stage, days 6 and 15 of adulthood. C. elegans exhibits increased susceptibility to bacterial infection with age. Results provide insight into molecular mechanisms underlying the decline in immune function with aging (immunosenescence).

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 3 age, 2 development stage sets

Platform:

GPL200

Series:

GSE21784

9 Samples

Download data: CEL

(Submitter supplied) Synchronized C. elegans cultures were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated at 25C for 4, 12 or 24 hours before harvesting. This experiment was repeated three times on independent occasions. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platforms:

GPL4208 GPL4206 GPL4207

18 Samples

Download data

(Submitter supplied) Analysis of genes differentially expressed between daf-2(e1368) and daf-2(e1368);pmk-1(km25) and between daf-2(e1368) and daf-2(e1368);daf-16(mgDf47). These studies identified genes upregulated by wild-type PMK-1 and wild-type DAF-16. Keywords: genetic modification

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3253

Platform:

GPL200

9 Samples

Download data: CEL

(Submitter supplied) Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: Time course, response to pathogen infection

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3252

Platform:

GPL200

18 Samples

Download data: CEL