GEO DataSets

Analysis of colon biopsies from individuals during acute E. histolytica diarrhea (day 1) versus convalescence (day 60) post metronidazole treatment. The E. histolytica pathogen causes diarrhea and colitis. Results provide insight into the molecular mechanisms underlying amebic colitis pathology.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 time sets

Platform:

GPL6244

Series:

GSE23750

16 Samples

Download data: CEL, CHP

(Submitter supplied) Differential expression was used to access gene differences after Entamoeba histolytica infection. Entamoeba histolytica is an important diarrheal pathogen worldwide, and induces apoptosis of the intestinal epithelium as part of its disease process. Regenerating (REG) 1 protein is anti-apoptotic. We investigated the involvement of REG 1 in E. histolytica colitis. Colonic biopsy samples were obtained from 8 subjects with acute E. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4374

Platform:

GPL6244

16 Samples

Download data: CEL, CHP

(Submitter supplied) A genome-wide transcriptional analysis of Entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture. Adaptation to the intestinal environment was accompanied by increases in a subset of cell signaling genes including transmembrane kinases, ras and rho family GTPases, and calcium binding proteins. Significant decreases in mRNA abundance for genes involved in glycolysis and concomitant increases in lipases were consistent with a change in energy metabolism. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

7 Samples

Download data: CEL

(Submitter supplied) A custom generated Affymetrix platform full genome microarray (E_his-1a520285F). This array has 7,712 unique probe sets representing 9,435 open reading frames (ORFs). Due to the highly repetitive nature of the E. histolytica genome, some of the probe sets are predicted to cross-hybridize with other sequences. Probe sets that represent a single gene are labeled as (_at). Probe sets in which at least one probe may cross-hybridize with another gene(s) are labeled as (_x_at). more...

Organism:

Entamoeba histolytica

10 Series

67 Samples

Download data: CDF

(Submitter supplied) Resistance to amebiasis is associated with a polymorphism in the leptin receptor. Previous studies demonstrated that humans with the ancestral Q223 leptin receptor allele were nearly four times less likely to be infected with Entamoeba histolytica than those carrying the mutant R223 allele. We hypothesized that the Q223 allele protected against E. histolytica via STAT3-mediated transcription of genes required for mucosal immunity. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

54 Samples

Download data: CEL, CHP

(Submitter supplied) The unicellular eukaryote Entamoeba histolytica is a human parasite that causes amebic dysentery and liver abscess. A genome-wide analysis of gene expression modulated by intestinal colonization and invasion identified an up-regulated transcript that encoded a putative high-mobility-group box (HMGB) protein, EhHMGB1. We tested if EhHMGB1 encoded a functional HMGB protein, and determined its role in control of parasite gene expression. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

6 Samples

Download data: CEL

(Submitter supplied) Insight on amoebiasis using a 3D model of the human intestine.

Organism:

Entamoeba histolytica HM-1:IMSS; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL26291

6 Samples

Download data: XLS

(Submitter supplied) We provided a full spectrum analysis for E. histolytica AGO2-2 associated 27nt small RNAs. Additionally, comparative analysis of small RNA populations from virulent and non-virulent amebic strains indicates that small RNA populations may regulate virulence genes.

Organism:

Entamoeba histolytica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16534

2 Samples

Download data: FA, TXT

(Submitter supplied) In order to dtermine the role of histone actylation in gene expresssion, E. histolytica 200:NIH trophozoites were treated with the HDAC inhibitor Trichostatin A, or grown in short chain fatty acids, which have been shown to alter histone actylation patterns in Entamoeba species. Keywords: Expression

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

8 Samples

Download data: CEL

(Submitter supplied) We discovered that colonization of the gut with the human bile acid 7'-dehydroxylating bacteria Clostridia scindens provided immunity to the parasite E. histolytica in a murine model, increased granulocyte monocyte progenitors, and that adoptive marrow transplant from C. scindens colonized mice into naive mice could recapitualte this protection.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

12 Samples

Download data: CSV