Testing the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes - - GEO DataSets

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Testing the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes

(Submitter supplied) The characterization of microbial community structure via 16S rRNA gene profiling has been greatly advanced in recent years by the application of amplicon pyrosequencing. The possibility of barcode-tagged sequencing of templates gives the opportunity to massively screen multiple samples from environmental or clinical sources for community details. However, an on-going debate questions the reproducibility and semi-quantitative rigour of pyrotag sequencing and, as in the early days of genetic community fingerprinting, pros and cons are continuously provided. more...

Organism:: environmental samples; environmental samples; aquifer metagenome; environmental samples; Aliivibrio fischeri

Type:: Expression profiling by high throughput sequencing; Other

Platforms:: GPL15201 GPL26069 GPL15200
21 Samples

Download data: FASTA, TXT

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Accession:: GSE35631

ID:: 200035631

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