GEO DataSet Links for PMC (Select 5888497) - GEO DataSets

Select item 2000923111.

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA Methylation by dCas9 methyltransferases (CRISPRme)

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:: GPL16791 GPL20795
17 Samples

Download data: BEDGRAPH, TXT

Series

Accession:: GSE92311

ID:: 200092311

PubMed Full text in PMC Similar studies

Select item 2000923102.

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA Methylation by dCas9 methyltransferases (CRISPRme) [WGBS]

(Submitter supplied) Fusion of active protein domains to the nuclease-deficient clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has been widely used for epigenome editing, but the specificities of these engineered proteins have still not been fully investigated. Targeted methylation of specific gene loci offers a direct approach to perturb DNA methylation-associated biological processes. more...

Organism:: Homo sapiens

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL20795
15 Samples

Download data: BEDGRAPH, TXT

Series

Accession:: GSE92310

ID:: 200092310

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000922613.

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA Methylation by dCas9 methyltransferases (CRISPRme) [ChIP-Seq]

(Submitter supplied) Fusion of active protein domains to the nuclease-deficient clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has been widely used for epigenome editing, but the specificities of these engineered proteins have still not been fully investigated. Targeted methylation of specific gene loci offers a direct approach to perturb DNA methylation-associated biological processes. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL16791
2 Samples

Download data: TXT

Series

Accession:: GSE92261

ID:: 200092261

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000749354.

Inhibition of uPA expression by CRISPR-dCas9 DNA methyltransferases

(Submitter supplied) We report the generation of CRISPR-dCas9 DNA methyltransferases to mediate targeted DNA methylation. Using the dCas9-BFP-DNMT3A and dCas9-BFP-DNMT3B methyltransferases, we have demonstrated that these two methyltransferase can mediate targeted methylation in three human genes tested: uPA, TGFBR3, and CDKN2A in human HEK293T cells. We also showed that these methyltransferases could mediate gene inhibition.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17303
5 Samples

Download data: TXT

Series

Accession:: GSE74935

ID:: 200074935

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

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