GEO DataSets

(Submitter supplied) We established the differentiation method of a limb bud organoid from mouse embryonic stem cells (mESCs) using SFEBq. mESCs-derived limb bud organoid selectively differentiate into forelimb or hindlimb by adjusting the retinoic acids activity. To evaluate a correlation of gene expression between limb bud organoid and embryonic tissues (limb bud, branchial arch, cardiac, and tail bud), we performed comparative transcriptome analysis using RNA-seq.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18480

27 Samples

Download data: CSV

(Submitter supplied) High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. We thus developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome these issues. Our improvements in several of the reaction steps of Quartz-Seq2 allow us to effectively convert initial reads to UMI counts (at a rate of 30%–50%). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19057 GPL17021

74 Samples

Download data: TXT

(Submitter supplied) Treatment with PARP inhibitor (PJ34) suppressed the formation of neurospheres by NSPCs of wild-type or Trp53-/- through the suppression of cell cycle progression and the induction of apoptosis. In order to identify the genes responsible for these effects.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

4 Samples

Download data: TXT