GEO DataSets

(Submitter supplied) Spores of Bacillales and Clostridiales species contain 100s of different mRNAs, and their major function in Bacillus subtilis is to provide ribonucleotides for new RNA synthesis when spores germinate. In new work, RNA was isolated from spores of five Bacillales and one Clostridioides species and relative spore mRNA levels were determined by RNA-seq. Determination of RNA levels in single spores allowed calculation of RNA nt/spore, and assuming mRNA is 3% of spore RNA allowed calculation that only ~6% of spore mRNAs were present at ≥ 1/spore. more...

Organism:

Bacillus atrophaeus ATCC 9372; Geobacillus stearothermophilus ATCC 12980; Bacillus thuringiensis str. Al Hakam; Clostridioides difficile 630; Priestia megaterium QM B1551; Bacillus subtilis

Type:

Expression profiling by high throughput sequencing

6 related Platforms

12 Samples

Download data: TXT

(Submitter supplied) CCA-adding enzymes are highly specific RNA polymerases that synthesize and maintain the sequence CCA at the tRNA 3‘-end. Here, we investigated the impact of cold adaptation on the reactivity and specificity of CCA-adding enzymes from psychrophilic bacteria. A comparative study of the corresponding enzymes from closely related psychro-, meso-, and thermophilic Bacillales indicates that the cold-adapted enzymes show a considerable error rate during CCA synthesis, resulting in additional incorporations of C and A residues. more...

Organism:

Geobacillus stearothermophilus; Bacillus subtilis subsp. subtilis str. 168; Planococcus halocryophilus; Exiguobacterium artemiae

Type:

Expression profiling by high throughput sequencing

4 related Platforms

23 Samples

Download data: CSV

(Submitter supplied) To investigate the transcriptional profile of GVE2 genes, the viral genes were identified by DNA microarray with Cy5- or Cy3-dUTP-labeled cDNAs prepared from uninfected and GVE2-infected Geobacillus sp. E263 at 4 h p.i.. After hybridization with the Cy3-dUTP-labeled cDNAs from GVE2-infected Geobacillus sp. E263 at 4 h p.i., Cy5-dUTP-labeled cDNAs from uninfected Geobacillus sp. E263 as well as Cy3- dUTP-labeled yeast cDNAs and Hex DNA, many spots produced positive signals significantly above the background, while no signal appeared for the Cy5-dUTP-labeled cDNAs from uninfected Geobacillus sp. more...

Organism:

Geobacillus sp. E263; Geobacillus virus E2

Type:

Expression profiling by array

Platform:

GPL4193

1 Sample

Download data: TIFF