GEO DataSets

(Submitter supplied) Extremely thermoacidophilic members of the Archaea such as the lithoautotroph, Metallosphaera sedula, are among the most acid resistant forms of life and are of great relevance in bioleaching. Here, adaptive laboratory evolution was used to enhance the acid resistance of this organism while genomics and transcriptomics were used in an effort to understand the molecular basis for this trait. Unlike the parental strain, the evolved derivative, M. more...

Organism:

Metallosphaera sedula

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21872

3 Samples

Download data: XLSX

(Submitter supplied) Thermomacidophilic archaea, such as Metallosphaera sedula, are lithoautotrophs that occupy metal-rich environments. In previous studies, a M. sedula mutant lacking the primary copper efflux transporter, CopA, became copper sensitive. In contrast, the basis for supra-normal copper resistance remained unclear in the spontaneous M. sedula mutant, CuR1. Here, transcriptomic analysis of copper-shocked cultures indicated that CuR1 had a unique regulatory response to metal challenge corresponding to up-regulation of 55 genes. more...

Organism:

Metallosphaera sedula DSM 5348; Metallosphaera sedula

Type:

Expression profiling by array

Platform:

GPL6785

5 Samples

Download data: GPR

(Submitter supplied) Metallosphaera sedula is an extremely thermoacidophilic archaeon that grows heterotrophically on peptides, and chemolithoautotrophically on hydrogen, sulfur, or reduced metals as energy sources. During autotrophic growth, carbon dioxide is incorporated into cellular carbon via the 3-hydroxypropionate /4-hydroxybutyrate cycle (3HP/4HB). To date, all of the steps in the pathway have been connected to enzymes encoded in specific ORFs, except for the one responsible for ligation of coenzyme A (CoA) to 4-hydroxybutyrate (4HB). more...

Organism:

Metallosphaera sedula; Metallosphaera sedula DSM 5348

Type:

Expression profiling by array

Platform:

GPL6785

3 Samples

Download data: GPR, TXT

(Submitter supplied) Thermoacidophilic archaea are found in heavy metal-rich environments and, in some cases, these microorganisms are causative agents of metal mobilization through cellular processes related to their bioenergetics. Given the nature of their habitats, these microorganisms must deal with the potentially toxic effect of heavy metals. Here, we show that two thermoacidophilic Metallosphaera species with nearly identical (99.99%) genomes differed significantly in their sensitivity and reactivity to uranium. more...

Organism:

Metallosphaera sedula; Metallosphaera prunae; Metallosphaera sedula DSM 5348

Type:

Expression profiling by array

Platform:

GPL6785

9 Samples

Download data: CSV

(Submitter supplied) Hydrogen served as a competitive inorganic energy source, impacting the CuFeS2 bioleaching efficiency of the extremely thermoacidophilic archaeon Metallosphaera sedula. Open reading frames encoding key terminal oxidase and electron transport chain components were triggered by CuFeS2. Evidence of heterotrophic metabolism was noted after extended periods of bioleaching, presumably related to cell lysis.

Organism:

Metallosphaera sedula

Type:

Expression profiling by array

Platform:

GPL6785

3 Samples

Download data: CSV

(Submitter supplied) To study mixotrophy, it is desirable to have an organism capable of growth in the presence and absence of both organic and inorganic carbon sources, as well as organic and inorganic energy sources. Metallosphaera sedula is an extremely thermoacidophilic archaeon which has been shown to grow in the presence of inorganic carbon and energy source supplements (autotrophy), organic carbon and energy source supplements (heterotrophy), and in the presence of organic carbon and inorganic energy source supplements. more...

Organism:

Metallosphaera sedula

Type:

Expression profiling by array

Platform:

GPL6785

7 Samples

Download data: CSV

(Submitter supplied) Abstract: The crenarchaeal order Sulfolobales collectively contains at least five major terminal oxidase complexes. Based on genome sequence information, all five complexes are found only in Metallosphaera sedula and Sulfolobus tokodaii, the two sequenced Sulfolobales capable of iron oxidization. While specific respiratory complexes in certain Sulfolobales have been characterized previously as proton pumps for maintaining intracellular pH and generating proton motive force (pmf), their contribution to sulfur and iron biooxidation has not been considered. more...

Organism:

Metallosphaera sedula

Type:

Expression profiling by array

Platform:

GPL6785

5 Samples

Download data: CSV

(Submitter supplied) (from abstract): Iron oxidation is a desirable trait of biomining microorganisms, although the mechanism is not well-understood in extreme thermoacidophiles. The complete genome sequence of the extremely thermoacidophilic archaeon Metallosphaera sedula DSM 5348 (2.2 Mb, ~2300 ORFs) provides insights into biologically catalyzed metal sulfide oxidation. Comparative genomics was used to identify pathways and proteins (in)directly involved with bioleaching. more...

Organism:

Metallosphaera sedula

Type:

Expression profiling by array

Platform:

GPL6785

1 Sample

Download data: CSV

(Submitter supplied) A DNA microarray was designed and constructed based on at least 2258 protein-coding sequences in the M. sedula genome obtained from the Department on Energy’s Joint Genomes Institute (http://genome.ornl.gov/microbial/msed). OligoArray 2.1 (Rouillard, 2003) was used to generate at least one 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). more...

Organism:

Metallosphaera sedula

7 Series

33 Samples

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