GEO DataSets

(Submitter supplied) Purpose: Global survey of the transcriptome in autophagy-deficient strains of D. discoideum Results: Our RNAseq analysis of ATG5‾, ATG5‾/12‾ and ATG5‾/12‾/16‾ cells revealed major transcriptional changes in a large number of genes in comparison to AX2 Conclusions: Our study presents a first detailed analysis of the transcriptome in autophagy-deficient strains of D. discoideum. The data were generated by RNA-seq technology. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26000

24 Samples

Download data: TSV

(Submitter supplied) A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was applied to study methylation patterns in Dictyostelium discoideum

Organism:

Dictyostelium discoideum

Type:

Other

Platform:

GPL30893

2 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

154 Samples

Download data: CSV

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type with bzpS-, mybD-, nfyA-, DDB_G0281091-, DDB_G0269374- and DDB_G0272386- mutant cells dedifferentiating in growth medium

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

42 Samples

Download data: TXT, XLS

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum by single cell RNAseq

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

4 Samples

Download data: CSV

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type and forG- (formin-) mutant cells dedifferentiating in growth medium

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

24 Samples

Download data: TXT, XLS

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum, with comparison to forward development

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

84 Samples

Download data: TXT, XLS

(Submitter supplied) Background During infection by intracellular pathogens, a highly complex interplay occurs between the infected cell trying to degrade the invader and the pathogen which actively manipulates the host cell to enable survival and proliferation. Many intracellular pathogens pose important threats to human health and major efforts have been undertaken to better understand the host-pathogen interactions that eventually determine the outcome of the infection. more...

Organism:

Dictyostelium discoideum AX4; Dictyostelium discoideum AX2

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24705 GPL26762

10 Samples

Download data: TXT

(Submitter supplied) Micro (mi)RNAs regulate gene expression in many eukaryotic organisms where they control diverse biological processes. Their biogenesis, from primary transcripts to mature miRNAs, have been extensively characterized in animals and plants, showing distinct differences between these phylogenetically distant groups of organisms. However, little is known about miRNA biogenesis in organisms whose evolutionary position is placed in between plants and animals and/or in unicellular organisms. more...

Organism:

Dictyostelium discoideum AX2

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL24705 GPL24706

16 Samples

Download data: TXT

(Submitter supplied) In many developmental systems, morphogenesis is coupled with dramatic changes in spatiotemporal gene expression, often orchestrated by the coordinated action of transcription factors. Development of the social soil amoebae Dictyostelium discoideum proceeds through a sequence of morphological and transcriptional changes, but the role of transcription factors in development is not well understood. GtaC, a GATA-type zinc-finger transcription factor, is essential for Dictyostelium development. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9379

26 Samples

Download data: TXT

(Submitter supplied) We report on the function of the D. dictyostelium argonaute proteins AgnA and AgnB in the silencing mechanism of the retrotransposon DIRS-1. By deep sequencing analysis we found that AgnA is involved in the accumulation of DIRS-1 siRNAs since in respective knockout strains siRNAs are strongly reduced. This was verified by northern blots. Reduction of siRNAs was accompanied by increased transcript accumulation and the appearance of an extrachromosomal single stranded DIRS-1 cDNA. more...

Organism:

Dictyostelium discoideum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15643

4 Samples

Download data: WIG

(Submitter supplied) Biological oscillations are observed at many levels of cellular organization. In the social amoebae Dictyostelium discoideum, starvation-triggered multicellular development is organized by periodic cAMP waves, which provide both chemoattractant gradients and developmental signals. We report that GtaC, a GATA transcription factor, exhibits rapid nucleocytoplasmic shuttling in response to cAMP waves. This behavior requires coordinated action of a nuclear localization signal and reversible G protein-coupled receptor (GPCR)-mediated phosphorylation. While both are required for developmental gene expression, receptor occupancy promotes nuclear exit of GtaC, which leads to a transient burst of transcription at each cAMP cycle. We demonstrate that this biological circuit, like an “edge trigger”, filters out high frequency signals and counts those admitted, thereby enabling cells to modulate gene expression according to the dynamic pattern of the external stimuli.

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9379

32 Samples

Download data: TAB

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila in comparison to uninfected cells was investigated using DNA microarrays. Investigation of a 48 h time course of infection revealed several clusters of co-regulated genes, an enrichment of preferentially up- or downregulated genes in distinct functional categories and also showed that most of the transcriptional changes occurred 24 h after infection. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

6 Samples

Download data: CSV

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila in comparison to uninfected cells was investigated using DNA microarrays. Investigation of a 48 h time course of infection revealed several clusters of co-regulated genes, an enrichment of preferentially up- or downregulated genes in distinct functional categories and also showed that most of the transcriptional changes occurred 24 h after infection. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

6 Samples

Download data: CSV

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila in comparison to uninfected cells was investigated using DNA microarrays. Investigation of a 48 h time course of infection revealed several clusters of co-regulated genes, an enrichment of preferentially up- or downregulated genes in distinct functional categories and also showed that most of the transcriptional changes occurred 24 h after infection. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

6 Samples

Download data: CSV

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila in comparison to uninfected cells was investigated using DNA microarrays. Investigation of a 48 h time course of infection revealed several clusters of co-regulated genes, an enrichment of preferentially up- or downregulated genes in distinct functional categories and also showed that most of the transcriptional changes occurred 24 h after infection. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

6 Samples

Download data: CSV

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila in comparison to uninfected cells was investigated using DNA microarrays. Investigation of a 48 h time course of infection revealed several clusters of co-regulated genes, an enrichment of preferentially up- or downregulated genes in distinct functional categories and also showed that most of the transcriptional changes occurred 24 h after infection. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

6 Samples

Download data: CSV

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila was investigated using DNA microarrays. A detailed analysis of the 24 h time point post infection was performed in comparison to three controls, uninfected cells and co-incubation with Legionella hackeliae and L. pneumophila DeltadotA. One hundred and thirty-one differentially expressed D. discoideum genes were identified as common to all three experiments and are thought to be involved in the pathogenic response. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

19 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

27 Samples

Download data: CSV, TXT

(Submitter supplied) Differential gene expression of Dictyostelium discoideum after infection with Legionella pneumophila was investigated using DNA microarrays. A detailed analysis of the 24 h time point post infection was performed in comparison to three controls, uninfected cells and co-incubation with Legionella hackeliae and L. pneumophila DeltadotA. One hundred and thirty-one differentially expressed D. discoideum genes were identified as common to all three experiments and are thought to be involved in the pathogenic response. more...

Organism:

Dictyostelium discoideum

Type:

Expression profiling by array

Platform:

GPL1972

4 Samples

Download data: TXT