GEO DataSets

(Submitter supplied) Molecular mechanisms underlying aging associated impairments in learning and long-term memory storage are poorly understood. Here we leveraged an identified motor neuron L7, mediating long-term sensitization of siphon-withdrawal reflex, a form of non-associative learning in sea slug Aplysia, to assess the impact of aging on transcriptional changes during learning. RNAseq analysis of single L7 motor neuron isolated following short-term or long-term sensitization training from 8,10 and 12 months old Aplysia corresponding to mature, late mature and senescent stages have identified progressive impairments in transcriptional plasticity during aging. more...

Organism:

Aplysia californica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21596

49 Samples

Download data: TXT

(Submitter supplied) The marine opisthobranch mollusk, Aplysia californica, is a powerful experimental system in cellular, molecular, and behavioral neuroscience as well as cell and evolutionary biology because of the distinctive organization of its nervous system, which makes it advantageous for cellular and comparative analysis of a variety of behaviors and learning and memory. Aplysia's large neurons allow examination of neuronal architecture of instinctive and learned behaviors at the level of single characterized cells and defined cellular compartments (e.g., synapses). more...

Organism:

Aplysia californica

Type:

Expression profiling by high throughput sequencing; Other

4 related Platforms

353 Samples

Download data: XLSX

(Submitter supplied) We investigated genome folding across the eukaryotic tree of life. We find four general manifestations of genome organization at chromosome-scale that each emerge and disappear repeatedly over the course of evolution. The submission represents chromosome-length Hi-C contact maps, architecture type and homolog separation analyses for 26 species across the tree of life, representing all subphyla of chordates, all 7 extant vertebrate classes, and 7 out of 9 major animal phyla, as well as plants and fungi.

Organism:

Arachis hypogaea; Agaricus bisporus; Branchiostoma lanceolatum; Xenopus laevis; Notamacropus eugenii; Pygocentrus nattereri; Cristatella mucedo; Clonorchis sinensis; Chiloscyllium punctatum; Saccharomyces cerevisiae; Drosophila melanogaster; Gallus gallus; Hypsibius dujardini; Lethenteron camtschaticum; Triticum aestivum; Caenorhabditis elegans; Aplysia californica; Aedes aegypti; Culex quinquefasciatus; Homo sapiens; Muntiacus reevesi; Muntiacus muntjak; Strongylocentrotus purpuratus; Ciona intestinalis; Pleurobrachia bachei; Acropora millepora; Python bivittatus

Type:

Other

30 related Platforms

32 Samples

Download data: BEDPE, FASTA, HIC, VCF, WIG

(Submitter supplied) We used a custom-designed microarray to characterize the transcriptional response that accompanies longt-term sensitization training in the marine mollusk Aplysia californica. We characterized transcription for a new memory (1 day after training), a forgotten memory (8 days after training) and for a long-term savings memory (8 days after training but 1 day after a reminder that re-instates the sensitization memory). more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL18666

24 Samples

Download data: TXT

(Submitter supplied) We used a custom-designed microarray and qPCR to characterize the transcriptional response that persists 7 days after long-term sensitization training in the marine mollusk Aplysia californica, a time-point at which recall has decayed but savings memory is still possible to induce. Aplysia were exposed to a 1-day unilateral LTS training protocol (4 rounds of noxious shock at 30 min intervals, each shock 10s of 1/2s off 1/2on 90mA 60-hz biphasic square-wave shock). more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL18666

8 Samples

Download data: TXT

(Submitter supplied) We used a custom-designed microarray and qPCR to characterize the persistent transcriptional response to long-term sensitization training in the marine mollusk Aplysia californica. Aplysia were exposed to a 1-day unilateral LTS training protocol (4 rounds of noxious shock at 30 min intervals, each shock 10s of 1/2s off 1/2on 90mA 60-hz biphasic square-wave shock). Training produced robust sensitization in all animals (measured as an increase in T-SWR response from baseline to 24h after training). more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL18666

8 Samples

Download data: TXT

(Submitter supplied) We used a custom-designed microarray and qPCR to characterize the persistent transcriptional response to long-term habituation training in the marine mollusk Aplysia californica. Aplysia were exposed to either 3 days of spaced long-term habituation training (10 rounds of stimulation/day; each round = 15 min brushing at a 10s ISI; 15 min rest between rounds); a matched set of controls was not exposed to any brushing (n = 8/group). more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL18666

8 Samples

Download data: TXT

(Submitter supplied) We used a custom-designed microarray and qPCR to characterize the rapid transcriptional response to long-term sensitization training in the marine mollusk Aplysia californica. Aplysia were exposed to repeated noxious shocks to one side of the body (4 ten-second shocks at 90mA, 30 min ISI), a procedure known to induce a transcription-dependent and long-lasting increase in reflex responsiveness that is restricted to the side of training. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL18666

8 Samples

Download data: TXT

(Submitter supplied) Despite the advances in our understanding of aging-associated behavioral decline, we know relatively little about how aging affect neural circuits that underlie specific behaviors. Specifically, we know little about how aging affect expression of genes in specific neural circuits. We have now addressed this problem by exploring a cholinergic neuron R15, an identified neuron of marine snail Aplysia. R15 is characterized by bursting action potentials and is implicated in reproduction, osmoregulation and locomotion.

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL17112

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL13815

24 Samples

Download data

(Submitter supplied) Whole genome transcriptional profiling is used to compare ESTs found in cell bodies and processes of Aplysia sensory neurons RNA samples derived from cell bodies or processes of Aplysia single cultured sensory neurons were hybridized to custom Aplysia EST microarrays.

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL13815

8 Samples

Download data: TXT

(Submitter supplied) Whole genome transcriptional profiling is used to compare ESTs found in cell bodies and processes of Aplysia motor neurons RNA samples derived from cell bodies or processes of Aplysia single cultured motor neurons were hybridized to custom Aplysia EST microarrays.

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL13815

8 Samples

Download data: TXT

(Submitter supplied) Whole genome transcriptional profiling is used to identify ESTs found in RNA co-immunoprecipitated from Aplysia CNS with antibodies against Aplysia Kinesin Heavy Chain (ApKHC) RNA derived from Aplysia CNS served as control in the opposite channel. Custom Aplysia EST array to probe the samples was designed and ordered from Agilent.

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL13815

8 Samples

Download data: TXT

(Submitter supplied) Specific mRNAs are transported from the cell body to synapses where their translation can modify communication of pre-existing synapses and induce formation of new synaptic connections in response to learning. Little is known, however, about the identity of the RNAs that are actively transported and when and how these RNAs are utilized during learning. By focusing on RNAs that are associated with kinesin, a motor protein that transports gene products from the cell body to synapses, we have now applied microarrays and 454 sequencing to identify actively transported RNAs from the Aplysia central nervous system. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platforms:

GPL3636 GPL3635

2 Samples

Download data: TXT

(Submitter supplied) The monitoring of global transcription patterns during development is a useful first step to understand mechanisms underlying growth, differentiation and patterning in a given species. However such large scale developmental studies are so far only available from a few selected model organisms such as mouse, Drosophila and the nematode C. elegans. Genomic scale information from the lophotrochozoa is now emerging. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platforms:

GPL3636 GPL3635

16 Samples

Download data: TIFF, TXT

(Submitter supplied) The complexity of events associated with age-related memory loss (ARML) cannot be overestimated. The problem is further complicated by the enormous diversity of neurons in the CNS and even synapses of one neuron within a neural circuit. Large-scale single-neuron analysis is not only challenging but mostly impractical for any model currently used in ARML. We simply do not know: do all neurons and synapses age differently or are some neurons (or synapses) more resistant to aging than others? What is happening in any given neuron while it undergoes “normal” aging? What are the genomic changes that make aging apparently irreversible? What would be the balance between neuron-specific vs global genome-wide changes in aging? In the proposed paper we address these questions and develop a new model to study the entire scope of genomic and epigenomic regulation in aging at the resolution of single functionally characterized cells and even cell compartments. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platforms:

GPL3636 GPL3635

33 Samples

Download data: TXT, XLS

(Submitter supplied) The marine mollusc Aplysia is a well established experimental system for cellular and systems neuroscience because of the relatively simple organization of its nervous system and the presence within it of the largest nerve cells in the animal kingdom, many of which are uniquely identifiable in every member of the species. Until now, molecular analyses of Aplysia have been seriously handicapped by lack of adequate genomic information, with only 200 sequences publicly available when this project was initiated in 2003. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platforms:

GPL3635 GPL3636

6 Samples

Download data: TIFF, TXT

(Submitter supplied) The marine mollusc Aplysia is a well established experimental system for cellular and systems neuroscience because of the relatively simple organization of its nervous system and the presence within it of the largest nerve cells in the animal kingdom, many of which are uniquely identifiable in every member of the species. Until now, molecular analyses of Aplysia have been seriously handicapped by lack of adequate genomic information, with only 200 sequences publicly available when this project was initiated in 2003. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL3636

3 Samples

Download data: TIFF, TXT

(Submitter supplied) The marine mollusc Aplysia is a well established experimental system for cellular and systems neuroscience because of the relatively simple organization of its nervous system and the presence within it of the largest nerve cells in the animal kingdom, many of which are uniquely identifiable in every member of the species. Until now, molecular analyses of Aplysia have been seriously handicapped by lack of adequate genomic information, with only 200 sequences publicly available when this project was initiated in 2003. more...

Organism:

Aplysia californica

Type:

Expression profiling by array

Platform:

GPL3635

3 Samples

Download data: TIFF, TXT