GEO DataSets

(Submitter supplied) To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

Organism:

Rhipicephalus microplus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33773

9 Samples

Download data: XLSX

(Submitter supplied) Control of complex parasites via vaccination remains challenging, with the current combination of vaccines and small drugs remaining the choice of control strategy. The development of new vaccines against complex endo- and ectoparasites remains challenging and more studies are providing evidence that multicomponent vaccines will be needed for the development of protective vaccines against parasites. more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL32975

6 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of midgut tissues isolated from Rhipicephalus microplus and Rhipicephalus decoloratus females at day 20 post infestation. This enabled the identification of transcripts that are species-specific or shared between the two tick species tested.

Organism:

Rhipicephalus microplus; Rhipicephalus decoloratus

Type:

Expression profiling by array

Platform:

GPL15231

4 Samples

Download data: GPR, TXT

(Submitter supplied) Transcriptional profiling of whole nymphs and larvae from Rhipicephalus microplus at day 4 and 7 post infestation, respectively. This enabled the identification of transcripts that are stage-specific or shared among the stages tested.

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL15231

8 Samples

Download data: GPR, TXT

(Submitter supplied) Transcriptional profiling of salivary gland, midgut and ovary tissues isolated from Rhipicephalus microplus females at day 20 post infestation. This enabled the identification of transcripts that are tissue-specific or shared among the tissues tested.

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL15231

12 Samples

Download data: GPR, TXT

(Submitter supplied) The NimbleGen cattle tick custom microarray based on the BmiGI.V2 database of R. microplus ESTs was used to screen the resulting mRNA harvested from ticks treated with the tick Ubiquitin-63E 600bp dsRNA in comparison to controls. A total of 144 ESTs including TC6372 (Ubiquitin-63E) were down-regulated with 136 ESTs up-regulated following treatment. The results obtained substantiated the knockdown phenotype with ESTs identified as associated with ubiquitin proteolysis as well as oogenesis, embryogenesis, fatty acid synthesis and stress responses. more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL10125

8 Samples

Download data: TXT

(Submitter supplied) In addressing R. microplus - A. marginale interactions, we propose and test three linked hypotheses. The first is that the tick gene response is organ specific: the midgut gene regulation is unique during feeding and during acquisition of A. marginale as compared to the salivary gland. This distinction is relevant as the two organs serve very different roles in the transmission biology of A. marginale with early survival and replication within the midgut epithelium, composed of highly phagocytic cells, required for initial colonization while a second round of replication in the salivary gland acini, composed of highly secretory cells, is required for transmission of an infectious dose in the saliva. more...

Organism:

Anaplasma marginale str. St. Maries; Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL10388

30 Samples

Download data: XYS

(Submitter supplied) Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and B. taurus cattle Global analysis of gene expression changes in R. microplus during larval, pre-attachment and early adult stages of its life cycle feeding on Bos indicus and Bos taurus cattle were compared using gene expression microarray analysis. more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL10125

10 Samples

Download data: TXT

(Submitter supplied) Babesia bovis exposed tissues: Three tissues were looked at. 1. Adult Female Gut 2. Adult Ovary 3. Larvae. In all there are 24 measurements for feature (EST), and 4 measurements per treatment for each of the 6 treatment groups. The 6 treatment groups are: Gut infected (GI), Gut control (GC), and similarly for Ovary and Larval: OI, OC, LI, LC. There are 12 chips, each with a spot replicate. more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL6373

24 Samples

Download data: FTR, TIFF, TXT

(Submitter supplied) Babesia infected (and control) ovary tissue was used to construct LongSAGE libraries. The intent was to find differential gene expression in both libraries. Details: The extraction was done using Ambion's ToTALLY RNA kit, and the concatemers where constructed using Invitrogen's I-SAGE Long kit. The concatmer files (multiple fasta sequence files) were processed using in-house perl scripts to extract the 17bp LongSAGE tags. more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by SAGE

Platform:

GPL6274

2 Samples

Download data: TXT

(Submitter supplied) A R. microplus microarray was used to study differential gene expression in acaricide exposed larvae from an amitraz-resistant strain. The acaricide treatments were: organophosphate (OP), pyrethroid, ivermectin, and amitraz. The microarrays contained over 13,000 probes corresponding to each member of R. microplus gene index ESTs previously described (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus). more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by array

Platform:

GPL6373

20 Samples

Download data: TXT

(Submitter supplied) Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial Analysis of Gene Expression (SAGE) was used to analyze differential gene expression in response to OP treatment and to compare the responses in OP-treated and untreated resistant and susceptible tick larvae. more...

Organism:

Rhipicephalus microplus

Type:

Expression profiling by SAGE

Platform:

GPL6351

4 Samples

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(Submitter supplied) Sureprint

Organism:

Rhipicephalus microplus

1 Series

6 Samples

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(Submitter supplied) B_microplus EST database Arrays of this design have barcodes that begin with 16024497 or 2524497. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Rhipicephalus microplus

3 Series

24 Samples

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(Submitter supplied) NimbleGen design name 090120_Amar_Bmic_RFMC, NimbleGen design ID 19181. A Roche NimbleGen high-density gene expression microarray custom designed for 14,447 genes based on the expressed sequence tag (EST) database, B. microplus Gene Index Version 2 (BmiGI V2) for R. microplus. Eight 60-mer probe per gene; each probe is replicated 1 time. The design includes random GC and other control probes. Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. more...

Organism:

Rhipicephalus microplus; Anaplasma marginale str. St. Maries

1 Series

30 Samples

Download data: NDF, NGD

(Submitter supplied) see www.nimblegen.com

Organism:

Rhipicephalus microplus

2 Series

1 Related Platform

18 Samples

Download data

(Submitter supplied) www.nimblegen.com

Organism:

Rhipicephalus microplus

2 Series

1 Related Platform

44 Samples

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(Submitter supplied) This is a virtual platform for SAGE libraries generated for Rhipicephalus microplus using NlaIII as an anchor enzyme.

Organism:

Rhipicephalus microplus

1 Series

4 Samples

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(Submitter supplied) This is a virtual platform for SAGE libraries generated for Rhipicephalus microplus using NlaIII as an anchor enzyme.

Organism:

Rhipicephalus microplus

1 Series

2 Samples

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