GEO DataSets

(Submitter supplied) Purpose: sRNA-sequencing of mature and intermediate gonadal tissue in order to identify the differential expression of miRNAs during male to female (i.e. protandrous) sex transition Methods: Total RNA was extracted and sRNA was purified. cDNA libraries were constructed using a high definition adapter protocol (Xu et al. 2015). 50 bp sequencing was performed on Illumina's HiSeq 2500 at the Earlham Institute, Norwich, UK. more...

Organism:

Lates calcarifer

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25830

22 Samples

Download data: FA, TXT

(Submitter supplied) In this study, the relative effect of consuming five different commercial feeds (Feed B-F) and and frozen fish (control; Feed A) for two months on our selected juvenile Asian seabass (77.3g ± 22.4g) were investigated. The growth performance, the biochemical analysis of their flesh, the histology of their midgut and the transcriptome of their midgut and liver were compared to each other and controls. more...

Organism:

Lates calcarifer

Type:

Expression profiling by array

Platform:

GPL17855

24 Samples

Download data: TXT

(Submitter supplied) In this study, we attempt to characterize the transcriptomic profile of the Asian seabass brains collected from the male and female sexes. The objective is to identify genes that show sexually dimorphic expression in the brain of this species. For this experiment, Asian seabass were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore. There were no treatments carried out in this experiment. more...

Organism:

Lates calcarifer

Type:

Expression profiling by array

Platform:

GPL17855

8 Samples

Download data: TXT

(Submitter supplied) In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2–4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. more...

Organism:

Lates calcarifer

Type:

Expression profiling by array

Platform:

GPL17855

22 Samples

Download data: TXT

(Submitter supplied) In this study we attempt to elucidate some of the pathways involved in the immune response to vaccination and subsequent disease challenge using a transcriptomic approach. We exposed, via intra-peritoneal injection, three months old Asian seabass (Lates calcarifer) to a Streptococcus iniae vaccine. A control group was also set up where no vaccine was injected. Spleen and head kidney samples were collected at one and seven days post vaccination for transcriptomic analysis. more...

Organism:

Lates calcarifer

Type:

Expression profiling by array

Platform:

GPL17855

57 Samples

Download data: TXT

(Submitter supplied) Cryptocaryon irritans is the causative agent of white spot disease that infects virtually all marine teleost including Lates calcarifer. We undertook to gain an informative snapshot of the host’s transcriptomic response during the course of infection. Liver and spleen cDNA libraries-based L. calcarifer cDNA microarray were used as a tool to oversee the infection response. Parasites infection was carried out on juvenile fish for four days, and total RNA was extracted from the daily harvested infected liver. more...

Organism:

Lates calcarifer

Type:

Expression profiling by array

Platform:

GPL15067

24 Samples

Download data: GPR

(Submitter supplied) Seabass_v3_with300Techreps_3 300 tech reps chosen from the 60K probe design and added in as a probe group (i.e. all probes for a sequence have the SAME probe ID) Arrays of this design have barcodes that begin with 16045004 or 2545004. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. more...

Organism:

Lates calcarifer

4 Series

111 Samples

Download data: TXT

(Submitter supplied) Using the GeneTac™G3 (Genomic Solutions™, USA) arrayer, we fabricated the in-house Asian seabass (Lates calacrifer) cDNA microarray slide, comprising 1920 probes sourced from in-house Lates calcarifer spleen and liver cDNA libraries. Putative functions encoded by these probes include immunity, stress response, metabolism, transport, transcription and translation. Negative controls, such as oligonucleotide (A)50 and several Saccharomyces cerevisiae and Escherichia coli genes, as well as positive controls, such as a serial dilution of L. more...

Organism:

Lates calcarifer

1 Series

24 Samples

Download data