GEO DataSets

(Submitter supplied) Sex differentiation is a complex process that requires many factors to regulate proliferation, differentiation, development, and organization of the gonads. In teleosts, the molecular mechanisms of sex differentiation are diverse and unclear, especially in hermaphrodite fish. In the present study, 15 cell types, including germ cells, follicle cells, and theca cells, and so on, were identified using single-cell RNA sequencing (scRNA-seq) in the gonads during sex differentiation in a hermaphrodite fish, orange-spotted grouper (Epinephelus coioides). more...

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32885

6 Samples

Download data: FA, MTX, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22973

12 Samples

Download data: TXT

(Submitter supplied) V. parahaemolyticus and E. coioides were served as research objects to explore the interaction mechanism between pathogen and host. infection. The mRNA-miRNA integrated analysis of the spleen of E. coioides infected with V. parahaemolyticus was performed, and several crucial pathways were screened, which may contribute to treatment of V. parahaemolyticus infection.

Organism:

Epinephelus coioides

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22973

6 Samples

Download data: TXT

(Submitter supplied) V. parahaemolyticus and E. coioides were served as research objects to explore the interaction mechanism between pathogen and host. infection. The mRNA-miRNA integrated analysis of the spleen of E. coioides infected with V. parahaemolyticus was performed, and several crucial pathways were screened, which may contribute to treatment of V. parahaemolyticus infection.

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22973

6 Samples

Download data: TXT

(Submitter supplied) Purpose:To study the molecular mechanism of nerve necrosis (VNN) virus infection, and to provide help for molecular breeding and further development of Marine fishery Results: we found that 17,939 genes are elevated after NNV infection compared with control group, suggesting that NNV infection can strongly induce the expression of immune associated genes. In summary, we obtained high quality transcriptome of orange-spotted grouper and comparative analysis of transcriptome analysis reveals dramatically perturbation of gene expression with NNV infection.

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26202

9 Samples

Download data: FASTA, TXT

(Submitter supplied) In order to obtain complete transcriptome data of epinephelus coioides

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22973

1 Sample

Download data: FA, TXT

(Submitter supplied) Cortisol was injected into the protogynous epinephelus coioides to investigate the role of this hormone on sex change. Following injection, we evaluated sex-related gene expression during the processes of cortisol-induced sex change in epinephelus coioides.

Organism:

Epinephelus coioides

Type:

Expression profiling by RT-PCR

Platform:

GPL27964

16 Samples

Download data: TXT

(Submitter supplied) To characterize the differentially expressed genes between infected skin with normal skin

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22973

9 Samples

Download data: FA, XLS

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized analysis of the immune response to V. alginolyticus in Epinephelus coioides larvae, we used high-throughput deep sequencing technology to study the effect of infection on gene expression. Methods: Epinephelus coioides larvae (30-days-old) were injected with V. alginolyticus , and fish were sacrificed at 24 h after infection. Ten wild-type Epinephelus coioides larvae were used to build an EST library for transcriptome analysis. more...

Organism:

Epinephelus coioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19400

3 Samples

Download data: FA, TXT

(Submitter supplied) microRNAs (miRNAs) are an evolutionarily conserved class of non-coding RNA molecules, which regulate kinds of biological processes at post-transcriptional level. Investigation of miRNAs expression profiles using high-throughput strategies is efficiently conductive to identify and characterize miRNAs. In this study, through Solexa deep sequencing approach, we obtained 115 orange spotted grouper (Epinephelus coioides) encoded miRNAs. more...

Organism:

Epinephelus coioides

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19578

1 Sample

Download data: TXT

(Submitter supplied) Singapore grouper iridovirus (SGIV) is the major agent that causes severe iridovirus diseases in grouper maricluture. Based on the genomic information, a DNA microarray, containing probes corresponding to 162 putative SGIV open reading frames (ORFs) was constucted. The viral microarrays wereused to classify the majority of SGIV transcripts into three temporal kinetic classes (immediate-early, IE; early, E; late, L) during an in vitro infection by their dependence on de novo protein synthesis inhibitor and viral DNA replication. more...

Organism:

Epinephelus coioides; Iridovirus

Type:

Expression profiling by array

Platform:

GPL4893

4 Samples

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(Submitter supplied) Singapore grouper iridovirus (SGIV) is the major agent that causes severe iridovirus diseases in grouper maricluture. Based on the genomic information, a DNA microarray, containing probes corresponding to 162 putative SGIV open reading frames (ORFs), was constructed to map the viral gene transcriptional profiles over the time course by establishing the models of SGIV-infected GS cells and SGIV-infected grouper. more...

Organism:

Iridovirus; Epinephelus tauvina; Epinephelus coioides

Type:

Expression profiling by array

Platform:

GPL4893

22 Samples

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(Submitter supplied) PCR assay Protocol: see manufacturer's website

Organism:

Epinephelus coioides

1 Series

16 Samples

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