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Platform GPL1743 Query DataSets for GPL1743
Status Public on Dec 21, 2004
Title Zebrafish nucleotide array
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Danio rerio
Description Microarrays of the MWG 14k Zebrafish Oligonucleotide Set were produced using the facilities of the Leiden Genome Technology Center ( Oligonucleotides were dissolved in 150mM phosphate buffer (pH 8.5) to a concentration of 20 µM. With Omnigrid 100 (Genemachines) oligonucleotides were spotted on Codelink activated slides (Amersham Biosciences) according to the Amersham Codelink protocol and as described in (‘t Hoen et al 2003 and Meijer et al 2004). Microarrays were spotted according to the MIAME guidelines with 15.532 unique zebrafish oligonucleotides from the MWG oligonucleotide set. Ambion’s arraycontrol oligonucleotides (8 sense oligos) and 3 custom designed oligonucleotides were spotted together with the MWG oligonucleotide set. Spotted Codelink activated slides were treated with blocking solution and washed according to the manufacturer’s instructions.
Contributor(s) Bagowski CP, van der Meer DL, Leito JT
Submission date Dec 10, 2004
Last update date Oct 28, 2005
Contact name Christoph Peter Bagowski
Phone ++31715274975
Organization name Leiden University
Street address
City Leiden
ZIP/Postal code 2333 AL
Country Netherlands
Samples (5) GSM37552, GSM37577, GSM37578, GSM37579, GSM37580
Series (1)
GSE2069 Differential expression under hypoxia in zebrafish

Data table header descriptions
GB_ACC Genebank accession number

Data table
Ambion_1 Ambion_1
Ambion_2 Ambion_2
Ambion_3 Ambion_3
Ambion_4 Ambion_4
Ambion_5 Ambion_5
Ambion_6 Ambion_6
Ambion_7 Ambion_7
Ambion_8 Ambion_8
Empty Empty
empty001 empty001
empty002 empty002
empty003 empty003
empty004 empty004
empty005 empty005
empty006 empty006
empty007 empty007
empty008 empty008
empty009 empty009
empty010 empty010
empty011 empty011

Total number of rows: 15174

Table truncated, full table size 453 Kbytes.

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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