|
| Status |
Public on Dec 30, 2021 |
| Title |
DNA methylation profiling of TF bound sites using ChIPBS (ChIP Bisulfite Sequencing) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
ChIPBS analysis of HepG2 cells was performed using more direct assay approach, to study the methylation status of transcription factor bound sites and elucidate our understanding on TF-DNA methylation crosstalk
|
| |
|
| Overall design |
ChIP followed by Bisulfite sequencing were performed in HepG2 cell lines. Methylation profile was measured for JUND, YY1, ZBTB33, POL2R and TAF1 and compared to the wgbs methylation profile for the same TF bound sites.
|
| |
|
| Contributor(s) |
Partridge EC, Chhetri SB, Myers RM, Mendenhall EM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 26, 2017 |
| Last update date |
Dec 31, 2021 |
| Contact name |
Eric M Mendenhall |
| E-mail(s) |
emendenhall@hudsonalpha.org
|
| Organization name |
HudsonAlpha Institute
|
| Lab |
Mendenhall Lab
|
| Street address |
601 Genome Way Northwest
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (14)
|
|
| This SubSeries is part of SuperSeries: |
| GSE104248 |
Towards an integrative map of a single cell type's transcription factor landscape |
|
| Relations |
| BioProject |
PRJNA412696 |
| SRA |
SRP119183 |
Less...
More...