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| Status |
Public on Dec 30, 2021 |
| Title |
input control TAF1, YY1 rep1 |
| Sample type |
SRA |
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| Source name |
HepG2 cell
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| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
chip antibody: none
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| Growth protocol |
HepG2 were grown according to the approved ENCODE cell culture protocols (http://hgwdev.cse.ucsc.edu/ENCODE/protocols/cell)
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. The eluted DNA was followed by bisulfite conversion using the EZ DNA Methylation-Gold Kit from Zymo according to manufacturer’s instructions.
Illumina Single End for ChIPBS
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| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
SL81124
ChIPBS (ChIP followed by bisulfite sequencing)
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| Data processing |
Reads were trimmed (2-3 bp from ends) using trim galore (version 0.3.7), powered by cutadapt to trim adaptor sequences for QC purpose
ChIPBS reads were processed with Bismark(bismark-0.11.1) pipeline using Bowtie2(bowtie-2-2.1.0) aligner
Deduplication and PCR duplicates removal was performed using deduplicate_bismark program
Methylation extraction from deduplicated bam files, in CpG context, was performed using bismark_methylation_extractor program
Genome_build: hg19
Supplementary_files_format_and_content: .bedGraph - [Chromosome number, start position, stop position, dataValue], .cov - [Chromosome number, start position, stop position, methylation percent, methylated reads, unmethylated reads]
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| Submission date |
Sep 26, 2017 |
| Last update date |
Dec 30, 2021 |
| Contact name |
Eric M Mendenhall |
| E-mail(s) |
emendenhall@hudsonalpha.org
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| Organization name |
HudsonAlpha Institute
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| Lab |
Mendenhall Lab
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| Street address |
601 Genome Way Northwest
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| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
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| Platform ID |
GPL16791 |
| Series (2) |
| GSE104246 |
DNA methylation profiling of TF bound sites using ChIPBS (ChIP Bisulfite Sequencing) |
| GSE104248 |
Towards an integrative map of a single cell type's transcription factor landscape |
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| Relations |
| BioSample |
SAMN07702241 |
| SRA |
SRX3216478 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2793716_SL81124_CpG_context_deduplicated.bedGraph.gz |
36.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSM2793716_SL81124_CpG_context_deduplicated.bismark.cov.gz |
32.8 Mb |
(ftp)(http) |
COV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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