|
| Status |
Public on Mar 16, 2008 |
| Title |
Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
ChIP-chip analyses of ClrC components across the S. pombe genome.
Keywords: ChIP-chip
|
| |
|
| Overall design |
Chromatin immunoprecipitated DNA recovered with antibody against FLAG-tagged protein from wild-type asynchronous growing fission yeast cells and whole cell extracts DNA were amplified by random priming and respectively labeled with Cy5 and Cy3. Labeled DNA samples were mixed and hybridized onto a 44k pombe Agilent oligo array. Data were processed using Agilent scanner and Feature Extraction software
|
| |
|
| Contributor(s) |
Zhang K, Mosch K, Fischle W, Grewal SI |
| Citation(s) |
18345014 |
| |
| Submission date |
Feb 19, 2008 |
| Last update date |
Sep 19, 2016 |
| Contact name |
Shiv Grewal |
| Phone |
2407607553
|
| Organization name |
NCI
|
| Department |
LBMB
|
| Lab |
Shiv Grewal
|
| Street address |
NCI bldg 37 Rm 6068 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL4682 |
pombe 44K_v2 |
| GPL6503 |
Agilent-016010 S.pombe 4X44K_v3 (Feature Number version) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA107815 |
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