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Series GSE131733 Query DataSets for GSE131733
Status Public on Dec 11, 2019
Title Transient genome-wide adaptations of nascent RNAPII transcription triggered by low temperature
Organism Arabidopsis thaliana
Experiment type Other
Summary We determined RNA polymerase (RNAPII) transcription at single-nucleotide resolution in Arabidopsis during early stages of exposure to low temperature. Our data inform on the dynamics of RNAPII transcription and detect thousands of often short-lived uncharacterized transcripts. We demonstrate that low temperature triggers transient genome-wide effects on RNAPII activity, in particular the stalling of RNAPII at both gene ends and the distance of read-through transcription.
Overall design Plant native elongating transcripts sequencing (plaNET-Seq) was used to determine the genomic positions of transcriptionally engaged RNAPII in Col-0 seedlings which were exposed to cold (4⁰C) for 0h, 3h or 12h.
Contributor(s) Kindgren P, Ivanov M, Marquardt S
Citation(s) 31863587, 34058980
Submission date May 23, 2019
Last update date Jun 09, 2021
Contact name Maxim Ivanov
Organization name University of Copenhagen
Department Dept. of Plant and Environmental Sciences
Street address Thorvaldsensvej 40
City Frederiksberg C
ZIP/Postal code 1871
Country Denmark
Platforms (2)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
Samples (13)
GSM3814845 s01_WT_RT_rep1
GSM3814846 s02_WT_RT_rep2
GSM3814847 s03_WT_Cold_3h_rep1
BioProject PRJNA544565
SRA SRP199385

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Supplementary file Size Download File type/resource
GSE131733_RAW.tar 373.9 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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