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| Status |
Public on Dec 11, 2019 |
| Title |
Transient genome-wide adaptations of nascent RNAPII transcription triggered by low temperature |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
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| Summary |
We determined RNA polymerase (RNAPII) transcription at single-nucleotide resolution in Arabidopsis during early stages of exposure to low temperature. Our data inform on the dynamics of RNAPII transcription and detect thousands of often short-lived uncharacterized transcripts. We demonstrate that low temperature triggers transient genome-wide effects on RNAPII activity, in particular the stalling of RNAPII at both gene ends and the distance of read-through transcription.
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| Overall design |
Plant native elongating transcripts sequencing (plaNET-Seq) was used to determine the genomic positions of transcriptionally engaged RNAPII in Col-0 seedlings which were exposed to cold (4⁰C) for 0h, 3h or 12h.
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| Contributor(s) |
Kindgren P, Ivanov M, Marquardt S |
| Citation(s) |
31863587, 34058980 |
| Submission date |
May 23, 2019 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Maxim Ivanov |
| E-mail(s) |
maxim.ivanov@plen.ku.dk
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| Organization name |
University of Copenhagen
|
| Department |
Dept. of Plant and Environmental Sciences
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| Street address |
Thorvaldsensvej 40
|
| City |
Frederiksberg C |
| ZIP/Postal code |
1871 |
| Country |
Denmark |
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| Platforms (2) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
| GPL21785 |
Illumina HiSeq 4000 (Arabidopsis thaliana) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA544565 |
| SRA |
SRP199385 |
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