NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE139315 Query DataSets for GSE139315
Status Public on Apr 17, 2021
Title Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle
Organism Mus musculus
Experiment type Expression profiling by array
Summary Muscle stem cells (satellite cells) are distributed throughout the body and have heterogeneous properties among muscles. However, functional topographical genes in satellite cells of adult muscle remain unidentified. Here, we show that expression of Homeobox-A (Hox-A) cluster genes accompanied with DNA hypermethylation of the Hox-A locus was robustly maintained in both somite-derived muscles and their associated satellite cells in adult mice, which recapitulates their embryonic origin. Somite-derived satellite cells were clearly separated from cells derived from cranial mesoderm in Hoxa10 expression. Hoxa10 inactivation led to genomic instability and mitotic catastrophe in somite-derived satellite cells in mice and human. Satellite cell–specific Hoxa10 ablation in mice resulted in a decline in the regenerative ability of somite-derived muscles, which were unobserved in cranial mesoderm–derived muscles. Thus, our results show that Hox gene expression profiles instill the embryonic history in satellite cells as positional memory, potentially modulating region-specific pathophysiology in adult muscles.
 
Overall design Skeletal muscles were harvested from male, 12-week-old mice (C57BL/6J).
 
Contributor(s) Yoshioka K, Ono Y
Citation(s) 34108202
Submission date Oct 24, 2019
Last update date Oct 22, 2021
Contact name Yusuke Ono
Organization name Kumamoto University
Department Institute of Molecular Embryology and Genetics
Street address 2-2-1 Honjo
City Kumamoto
State/province Kumamoto
ZIP/Postal code 860-0811
Country Japan
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (9)
GSM4137995 MAS
GSM4137996 DIG
GSM4137997 TRP
Relations
BioProject PRJNA579280

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139315_RAW.tar 71.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap