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| Status |
Public on Apr 17, 2021 |
| Title |
Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Muscle stem cells (satellite cells) are distributed throughout the body and have heterogeneous properties among muscles. However, functional topographical genes in satellite cells of adult muscle remain unidentified. Here, we show that expression of Homeobox-A (Hox-A) cluster genes accompanied with DNA hypermethylation of the Hox-A locus was robustly maintained in both somite-derived muscles and their associated satellite cells in adult mice, which recapitulates their embryonic origin. Somite-derived satellite cells were clearly separated from cells derived from cranial mesoderm in Hoxa10 expression. Hoxa10 inactivation led to genomic instability and mitotic catastrophe in somite-derived satellite cells in mice and human. Satellite cell–specific Hoxa10 ablation in mice resulted in a decline in the regenerative ability of somite-derived muscles, which were unobserved in cranial mesoderm–derived muscles. Thus, our results show that Hox gene expression profiles instill the embryonic history in satellite cells as positional memory, potentially modulating region-specific pathophysiology in adult muscles.
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| Overall design |
Skeletal muscles were harvested from male, 12-week-old mice (C57BL/6J).
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| Contributor(s) |
Yoshioka K, Ono Y |
| Citation(s) |
34108202 |
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| Submission date |
Oct 24, 2019 |
| Last update date |
Oct 22, 2021 |
| Contact name |
Yusuke Ono |
| Organization name |
Kumamoto University
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| Department |
Institute of Molecular Embryology and Genetics
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| Street address |
2-2-1 Honjo
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| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA579280 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE139315_RAW.tar |
71.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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