|
| Status |
Public on Apr 17, 2021 |
| Title |
RF |
| Sample type |
RNA |
| |
|
| Source name |
Male, 12-week-old, C57BL/6J
|
| Organism |
Mus musculus |
| Characteristics |
Sex: Male
age: 12-week-old
strain: C57BL/6J
muscle tissue: rectus femoris
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested from freshly frozen muscle tissue using IsogenII reagent. The RNA was further purified with the Qiagen RNeasy Mini Kit (Qiagen) following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Following fragmentation, 5.5 ug of cDNA were hybridized on the GeneChip Mouse Gene 2.0 ST Array using the GeneChip Hybridization Oven 645. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
|
| |
|
| Hybridization protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Scan protocol |
The data were analyzed with Expression Console v1.4(Affymetrix) and Transcriptome Analysis Console v3.1 (Affymetrix). RMA was used as summarization algorithm.
|
| Data processing |
log2 GC-RMA signal
|
| |
|
| Submission date |
Oct 24, 2019 |
| Last update date |
Apr 17, 2021 |
| Contact name |
Yusuke Ono |
| Organization name |
Kumamoto University
|
| Department |
Institute of Molecular Embryology and Genetics
|
| Street address |
2-2-1 Honjo
|
| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE139315 |
Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle |
|
