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Series GSE140321 Query DataSets for GSE140321
Status Public on Nov 13, 2020
Title Distal regulation of c-myb expression in human myeloid leukemia cells
Organism Homo sapiens
Experiment type Other
Summary The transcription factor c-Myb a key regulator in proliferation and differentiation of hematopoietic progenitor cells, is precisely regulated during essential cellular processes. Overexpression and rearrangement of c-myb has been reported in human tumors including myeloid leukemia, but exact regulatory mechanisms have remained elusive. Here, we identified, using 4C assay with the c-myb promoter as an anchor, the interaction site at -34k region upstream of c-myb gene that are involved in c-myb expression. Furthermore, we found that the long-range interactions changed with c-myb being down-regulated on differentiation progress in human myeloid leukemia cell lines. Taken together, our date revealed that a potential c-myb enhancer-promoter interactions may be a particularly important regulatory mechanism for c-myb gene expression in human myeloid leukemia cells.
Overall design We used human myeloid leukemia cell lines K562, U937 and HL60 to carry out 4C-seq experiments, as a control, we carried out parallel 4C measurements in HeLa cells, the c-myb promoter fragment served as a bait fragment to detect the long-range interactions. To validate the presence of potential enhancers in Hbs1l-Myb intergenic region that regulate c-myb transcription by physically interacting with the c-myb promoter via chromatin looping, 4C-Seq was subsequently carried out using the c-myb promoter as viewpoint in K562, U937 and HL60 cells before and after differentiation, representing stages of high and low c-myb expression, respectively.
Contributor(s) Li M, Jiang P, Han B, Zhang J
Citation(s) 33637692
Submission date Nov 13, 2019
Last update date Mar 01, 2021
Contact name Mengjia Li
Organization name Shanghai Ocean University
Department College of Fisheries and Life Science
Lab Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources
Street address 999 Hucheng Ring Road
City Shanghai
ZIP/Postal code 201306
Country China
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (10)
GSM4159250 K562_myb_4C
GSM4159251 U937_myb 4C
GSM4159252 HL60_myb 4C
BioProject PRJNA589290
SRA SRP229733

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Supplementary file Size Download File type/resource
GSE140321_RAW.tar 3.5 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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