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| Status |
Public on Nov 13, 2020 |
| Title |
Distal regulation of c-myb expression in human myeloid leukemia cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
The transcription factor c-Myb a key regulator in proliferation and differentiation of hematopoietic progenitor cells, is precisely regulated during essential cellular processes. Overexpression and rearrangement of c-myb has been reported in human tumors including myeloid leukemia, but exact regulatory mechanisms have remained elusive. Here, we identified, using 4C assay with the c-myb promoter as an anchor, the interaction site at -34k region upstream of c-myb gene that are involved in c-myb expression. Furthermore, we found that the long-range interactions changed with c-myb being down-regulated on differentiation progress in human myeloid leukemia cell lines. Taken together, our date revealed that a potential c-myb enhancer-promoter interactions may be a particularly important regulatory mechanism for c-myb gene expression in human myeloid leukemia cells.
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| Overall design |
We used human myeloid leukemia cell lines K562, U937 and HL60 to carry out 4C-seq experiments, as a control, we carried out parallel 4C measurements in HeLa cells, the c-myb promoter fragment served as a bait fragment to detect the long-range interactions. To validate the presence of potential enhancers in Hbs1l-Myb intergenic region that regulate c-myb transcription by physically interacting with the c-myb promoter via chromatin looping, 4C-Seq was subsequently carried out using the c-myb promoter as viewpoint in K562, U937 and HL60 cells before and after differentiation, representing stages of high and low c-myb expression, respectively.
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| Contributor(s) |
Li M, Jiang P, Han B, Zhang J |
| Citation(s) |
33637692 |
| |
| Submission date |
Nov 13, 2019 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Mengjia Li |
| E-mail(s) |
mengjia.li@outlook.com
|
| Organization name |
Shanghai Ocean University
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| Department |
College of Fisheries and Life Science
|
| Lab |
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources
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| Street address |
999 Hucheng Ring Road
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| City |
Shanghai |
| ZIP/Postal code |
201306 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA589290 |
| SRA |
SRP229733 |
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