Other Genome binding/occupancy profiling by high throughput sequencing
Summary
INO80C chromatin remodeler colocalizes to yeast replication origins together with the transcription factors Mot1 and NC2. INO80C maintains genome stability by preventing noncoding transcription around origins both in yeast and mammals.
Overall design
Native elongating transcript sequencing (NET-seq) in WT-AA, INO80-AA, Mot1-AA, INO80-AA Mot1-AA; Break-seq in WT-AA, INO80-AA, Mot1-AA, INO80-AA Mot1-AA, ChIP-seq for Orc5-AA and Arp5-AA