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Series GSE14436 Query DataSets for GSE14436
Status Public on Dec 01, 2009
Title P16INK4a mediated supperssion of telomerase in normal and malignant human breast cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary The cyclin-dependent kinase inhibitor p16INK4a (CDKN2A) is an important tumor-suppressor gene frequently inactivated in human tumors. p16 suppresses the development of cancer by triggering an irreversible arrest of cell proliferation termed cellular senescence. Here, we describe another anti-oncogenic function of p16 in addition to its ability to halt cell cycle progression. We show that transient expression of p16 stably represses the hTERT gene, encoding the catalytic subunit of telomerase, in both normal and malignant breast epithelial cells. Short-term p16 expression increases the amount of histone H3 trimethylated on lysine 27 (H3K27) bound to the hTERT promoter, resulting in transcriptional silencing, likely mediated by polycomb complexes. Our results indicate that transient p16 exposure may prevent malignant progression in dividing cells by irreversible repression of genes, such as hTERT, whose activity is necessary for extensive self-renewal.
 
Overall design 27 samples were analyzed to study three histone modifications (H3K4me3, H3K9me3, H3K27me3) in three different cell lines. Each combination of histone modification and cell line had three biological replicates (A,B,C).
 
Contributor(s) Bazarov AV, Hines C, Lee L, Bassett E, Beliveau A, Campeau E, Mukhopadhyay R, Lee W, Melodyev S, Zaslavsky Y, Rodier F, Benhattar J, Ren B, Campisi J, Yaswen P
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Submission date Jan 14, 2009
Last update date Mar 20, 2012
Contact name Bing Ren
E-mail(s) biren@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL1454 RenLab ENCODE PCR tiling array
Samples (27)
GSM359288 H3K27me3 Yaswen1A RenLab ENCODE PCR tiling array replicate 1 llee_enc_H3K27me3_Yaswen1A_031608.gpr
GSM359289 H3K27me3 Yaswen1B RenLab ENCODE PCR tiling array replicate 1 llee_enc_H3K27me3_Yaswen1B_050108.gpr
GSM359290 H3K27me3 Yaswen1C RenLab ENCODE PCR tiling array replicate 1 llee_enc_H3K27me3_Yaswen1C_050808.gpr
Relations
BioProject PRJNA111771

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14436_RAW.tar 66.1 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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