 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 16, 2021 |
| Title |
The m6A reader CPSF30-L regulates alternative polyadenylation affecting nitrate signaling in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
As the most ubiquitous internal modification of eukaryotic mRNA, m6A (N6-methyladenosine) modification plays a vital role in almost every aspect of mRNA metabolism. However, the evidence of m6A in regulating the alternative polyadenylation (APA) is limited. APA is controlled by a large protein-RNA complex with many components including CPSF30. Arabidopsis CPSF30 has two isoforms, the longer one named CPSF30-L containing an additional YTH (YT512-B Homology)-domain in the C terminus, which is unique in plant. In this study, we revealed that m6A modification directly regulated APA, by proving the capability of Arabidopsis CPSF30-L YTH domain binding to m6A substrate by using in vitro assay and structural studies. We observed that extensive genes shifted their poly(A) sites in cpsf30-2 and found that numerous genes altered polyadenylation site (PAS) that correlated well with previously identified m6A peaks, indicating that genes carry m6A modification are prone to be regulated by APA. Moreover, we found that several important genes involved in nitrate metabolism are amongst those genes with APA site alteration in cpsf30-2.And we could rescue these APA and nitrate metabolism defects by introducing the wild-type CPSF30-L, but not by m6A-binding defective mutants (W259A, W310A or Y319A), which explained the nitrogen signaling defects of cpsf30-2 discovered previously. Taken together, our results demonstrated that m6A modification could directly regulate APA in Arabidopsis and revealed the function of m6A reader CPSF30-L in nitrate signaling by controlling APA regulation. This study will shed new lights on the roles of m6A modification during RNA 3’-end processing in plant development.
|
| |
|
| Overall design |
Investigate the role of AtCPSF30 in Alternative Polyadenylation
|
| |
|
| Contributor(s) |
Hou Y, Sun J, Cao X |
| Citation(s) |
33515769 |
| Submission date |
Feb 11, 2020 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Jing Sun |
| Organization name |
Chinese Academy of Sciences
|
| Department |
IGDB
|
| Street address |
No. 1 West Beichen Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platforms (2) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA606046 |
| SRA |
SRP248245 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145109_RAW.tar |
782.8 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...