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| Status |
Public on Jun 01, 2020 |
| Title |
A dynamic pattern of local auxin sources is required for root regeneration |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Following removal of its stem cell niche, the root meristem can regenerate by recruitment of remnant cells from the stump. Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown. Here, we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process. Auxin synthesis is highly localized and PIN-mediate transport is dispensable for auxin accumulation and tip regeneration. Roots lacking the activity of the regeneration competence factor ERF115, or that are dissected at a zone of low-regeneration potential, fail to activate local auxin sources. Remarkably, restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues. We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise spatio-temporal pattern.
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| Overall design |
Untreated root meristems control + root meristems treated with 100uM L-Kyn for 3h and 6h. Experiment were done for both uncut and for meristems dissected at 120um above the QC
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| Contributor(s) |
Matosevich R, Cohen I, Gil-Yarom N, Modrego A, Verna C, Scarpella E, Efroni I |
| Citation(s) |
32747761 |
| |
| Submission date |
Feb 18, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Idan Efroni |
| E-mail(s) |
idan.efroni@mail.huji.ac.il
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| Organization name |
Hebrew University
|
| Department |
Plant Sciences
|
| Street address |
POB 12
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| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA607298 |
| SRA |
SRP249854 |
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