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Series GSE145488 Query DataSets for GSE145488
Status Public on Jun 01, 2020
Title A dynamic pattern of local auxin sources is required for root regeneration
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Following removal of its stem cell niche, the root meristem can regenerate by recruitment of remnant cells from the stump. Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown. Here, we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process. Auxin synthesis is highly localized and PIN-mediate transport is dispensable for auxin accumulation and tip regeneration. Roots lacking the activity of the regeneration competence factor ERF115, or that are dissected at a zone of low-regeneration potential, fail to activate local auxin sources. Remarkably, restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues. We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise spatio-temporal pattern.
Overall design Untreated root meristems control + root meristems treated with 100uM L-Kyn for 3h and 6h. Experiment were done for both uncut and for meristems dissected at 120um above the QC
Contributor(s) Matosevich R, Cohen I, Gil-Yarom N, Modrego A, Verna C, Scarpella E, Efroni I
Citation(s) 32747761
Submission date Feb 18, 2020
Last update date Aug 31, 2020
Contact name Idan Efroni
Organization name Hebrew University
Department Plant Sciences
Street address POB 12
City Rehovot
ZIP/Postal code 76100
Country Israel
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (18)
GSM4319061 Mock_Uncut_1
GSM4319062 Mock_Uncut_2
GSM4319063 Mock_Uncut_3
BioProject PRJNA607298
SRA SRP249854

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Supplementary file Size Download File type/resource
GSE145488_matosevich_etal.csv.gz 492.7 Kb (ftp)(http) CSV
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