|
Status |
Public on Jun 01, 2020 |
Title |
Lkyn_Uncut_3_3 |
Sample type |
SRA |
|
|
Source name |
Apical root meristem
|
Organism |
Arabidopsis thaliana |
Characteristics |
treatment: L-kyn 3h
|
Treatment protocol |
For L-Kyn treatments, 7 days old seedlings were
|
Growth protocol |
Seedslings were germinated on 1/2MS agar plates containing 0.5% sucrose. Following 2 days at 4c, plates were move to a 22c for 16h light/8h dark cycle for 7 days
|
Extracted molecule |
total RNA |
Extraction protocol |
Root tips were flash frozen, grinded and RNA extracted using RNeasy micro-kit (Qiagen) according to the manufacturer instructions sequencing libraries prepared using Lexigen 3’ Quant-Seq kit, according to the manufacturer instructions.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
Matosevich_etal.csv
|
Data processing |
Reads were aligned to the genome using Bowtie2, followed by readcount using htseq-count Expression data were normalized using Deseq2 Genome_build: Arabidopsis thaliana TAIR10 Supplementary_files_format_and_content: Normalized expression reads for all replicates
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|
|
Submission date |
Feb 18, 2020 |
Last update date |
Jun 01, 2020 |
Contact name |
Idan Efroni |
E-mail(s) |
idan.efroni@mail.huji.ac.il
|
Organization name |
Hebrew University
|
Department |
Plant Sciences
|
Street address |
POB 12
|
City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
|
|
Platform ID |
GPL19580 |
Series (1) |
GSE145488 |
A dynamic pattern of local auxin sources is required for root regeneration |
|
Relations |
BioSample |
SAMN14127712 |
SRA |
SRX7740849 |