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| Status |
Public on May 26, 2021 |
| Title |
Transcriptome analysis of Arabidopsis thaliana shoots and roots, exposed to PEG-induced osmotic shock, PEG+Histidine, PEG+SHAM [20190311_172119_Peg_SHAMf1_res] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to better understand the context of root water stress and its relationship to other stresses, I undertook an evaluation of osmotic water stress by transcriptome analyses. Whole transcriptome MARSeq (Diego Adhemar Jaitin, 2017) analysis can enable us to study the changes in gene expression (changes in transcript prevalence) in depth under different treatments. It is a cost effective method to develop transcriptional activity. It uses relatively small amounts of RNA and reads sequences from the 3’-polyA containing end. This method can reveal underlying trends that can help us understand the less obvious processes happening in our system. To carry this out I processed whole transcriptome data separately from WT seedling roots exposure to PEG, and from roots under 30% PEG + 5mM HIS, 30% PEG + 1mM SHAM for 0, 1, 2, 3 h.
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| Overall design |
WT Arabidopsis thaliana seedlings were exposed to 30% PEG + 5mM HIS, 30% PEG + 1mM SHAM 0, 1, 2, 3 h, and root RNA was extracted sequenced using the MARSeq protocol.
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| Contributor(s) |
Chen T, Fluhr R |
| Citation(s) |
33793947 |
| |
| Submission date |
Jun 15, 2020 |
| Last update date |
May 26, 2021 |
| Contact name |
Tomer Chen |
| E-mail(s) |
tomerlivechen@gmail.com
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| Organization name |
Weizmann Institute of Science
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| Department |
Plant and Environmental Sciences
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| Street address |
P.O.box 26
|
| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE152462 |
A role for lipoxygenase in the production of 1O2 and promoting the root's response to osmotic stress in Arabidopsi |
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| Relations |
| BioProject |
PRJNA639497 |
| SRA |
SRP267330 |
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