NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE167842 Query DataSets for GSE167842
Status Public on Dec 01, 2021
Title Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size
Organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Biosynthesis scales with cell size such that protein concentrations generally remain constant as cells grow. As an exception, synthesis of the cell-cycle inhibitor Whi5 ‘sub-scales’ with cell size so that its concentration is lower in larger cells to promote cell-cycle entry. Here, we find that a transcriptional control uncouples Whi5 synthesis from cell size and, screening for similar genes, identify histones as the major class of sub-scaling transcripts besides WHI5. Histone synthesis is thereby matched to genome content rather than cell size. Such sub-scaling proteins are challenged by asymmetric cell division because proteins are typically partitioned in proportion to new-born cell volume. To avoid this fate, Whi5 uses chromatin-binding to partition similar protein amounts to each new-born cell regardless of cell size. Finally, disrupting both Whi5 synthesis and chromatin-based partitioning compromises G1 size control. Thus, specific transcriptional and partitioning mechanisms determine protein sub-scaling to control cell size.
 
Overall design ChIP-seq and RNA-seq libraries associated with the project
 
Contributor(s) Swaffer MP, Chandler-Brown D, Kim J, Langhinrichs M, Marinov GK, Greenleaf WJ, Kundaje A, Schmoller KM, Skotheim JM
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 26, 2021
Last update date Dec 03, 2021
Contact name Georgi Kolev Marinov
Organization name STANFORD UNIVERSITY
Department Genetics
Street address 279 Campus Drive West, Beckman Center, B-257A/259
City Stanford
State/province California
ZIP/Postal code 94305-5101
Country USA
 
Platforms (2)
GPL27812 Illumina NovaSeq 6000 (Saccharomyces cerevisiae)
GPL29170 Illumina NovaSeq 6000 (Saccharomyces cerevisiae; Schizosaccharomyces pombe)
Samples (72)
GSM5113889 190722L68_MS522_SWI4-V5-ChIP
GSM5113890 190722L69_MS524_SWI6-V5-ChIP
GSM5113891 191113L01_MS534_GFP-NLS-5xFLAG-ChIP
Relations
BioProject PRJNA705222
SRA SRP308424

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE167842_RAW.tar 2.9 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap