|
| Status |
Public on Jul 18, 2021 |
| Title |
scRNA-seq of stromal vascular fraction (SVF) of lipoma and bulk RNA-seq of lipoma-derived adipocytes. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We determined the gene signatures of adipocytes differentiated from lipoma SVF. Furthermore, single-cell RNA-sequencing was performed for SVF from inherited (familial) and sporadic lipoma.
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| |
|
| Overall design |
Patients undergoing surgical excision of lipomas were identified and the dermatologist clinically confirmed the diagnosis of lipoma. Tissues was enzymaticaly digested and SVF was obtained and diffrentiated in adipogenic media for 5-7 days before collection of the RNA.
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| |
|
| Contributor(s) |
Shinoda K, Deutsch A |
| Citation(s) |
35271854 |
| |
| Submission date |
Jul 11, 2021 |
| Last update date |
Mar 13, 2023 |
| Contact name |
Kosaku Shinoda |
| E-mail(s) |
bonitoo@gmail.com
|
| Phone |
7186781189
|
| Organization name |
Albert Einstein College of Medicine
|
| Lab |
Shinoda lab
|
| Street address |
1301 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (8)
|
| GSM5436521 |
Lipoma-derived adipocytes familial replicate2 |
| GSM5436522 |
Lipoma-derived adipocytes familial replicate3 |
| GSM5436523 |
Lipoma-derived adipocytes sporadic replicate1 |
| GSM5436524 |
Lipoma-derived adipocytes sporadic replicate2 |
| GSM5436525 |
Lipoma-derived adipocytes sporadic replicate3 |
|
| Relations |
| BioProject |
PRJNA745464 |
| SRA |
SRP327858 |
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