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Series GSE18440 Query DataSets for GSE18440
Status Public on Feb 16, 2010
Title Nuclear retention of fission yeast dicer is a prerequisite for RNAi-mediated heterochromatin assembly
Organism Schizosaccharomyces pombe
Experiment type Non-coding RNA profiling by genome tiling array
Summary RNAseIII ribonucleases act at the heart of RNA silencing pathways by processing precursor RNAs into mature microRNAs and siRNAs. In the fission yeast Schizosaccharomyces pombe, siRNAs are generated by the RNAseIII enzyme Dcr1 and are required for heterochromatin formation. In this study, we have analyzed the subcellular localization of Dcr1 and found that it accumulates in the nucleus and is enriched at the nuclear periphery. Nuclear accumulation of Dcr1 depends on a short motif which constrains nuclear export promoted by the double-stranded RNA binding domain of Dcr1. Absence of this motif renders Dcr1 mainly cytoplasmic and is accompanied by remarkable changes in gene expression and failure to assemble heterochromatin. Our findings suggest that dicer proteins are shuttling proteins and that the steady-state subcellular levels can be shifted towards either compartment. This has implications for the mechanism of RNAi-mediated heterochromatin assembly and the spatial organization of RNA silencing pathways in general.
 
Overall design 25 chips representing 6 different strains. Every condition is represented by at least two replicates
 
Contributor(s) Emmerth S, Schober H, Gaidatzis D, Jacobeit K, Bühler M
Citation(s) 20152181
Submission date Oct 06, 2009
Last update date May 27, 2014
Contact name Stephan Emmerth
E-mail(s) marc.buehler@fmi.ch
Organization name FMI
Lab Bühler
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL7715 [Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array
Samples (25)
GSM459494 wt tiling array 1
GSM459495 wt tiling array 2
GSM459496 wt tiling array 3
Relations
BioProject PRJNA118241

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18440_RAW.tar 254.1 Mb (http)(custom) TAR (of CEL)
GSE18440_expressionSet_RMA.txt.gz 1.0 Mb (ftp)(http) TXT
Processed data are available on Series record

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