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| Status |
Public on Feb 16, 2010 |
| Title |
wt tiling array 6 |
| Sample type |
RNA |
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| Source name |
wt total RNA
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| Organism |
Schizosaccharomyces pombe |
| Characteristics |
strain: SPB80
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| Growth protocol |
Cells were collected at OD600=0.5
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the hot phenol method (Leeds, 1991): resuspend cell pellet in 1.2 ml AE Buffer (50mM NaOAc pH 5.2, 10mM EDTA), add 80 ml 25 % SDS and 1.2 ml acidic phenol, incubate for 10 minutes at 65 °C and then put on ice for 5 minutes transfer to 15 ml phase lock Falcon tubes, add 1.3 ml chloroform, mix by inverting tube, spin 10 minutes 3000 rpm at room temperature, pour the supernatant into a 15 ml Falcon tube, add 3 ml glycogen, 4 ml 100 % ethanol and 160 ml 3M NaOAc pH 5.2-5.6, spin 30 minutes 12000 rpm at 4°C, wash 2 times with 70% ethanol, air dry pellet, resuspend in 500 ul water.
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| Label |
biotin
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| Label protocol |
Samples (200 ng of DNA) were enzymatically fragmented and labeled according to the GeneChip® Whole Transcript(WT) Double-Stranded Target Assay
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| Hybridization protocol |
Affymetrix protocol
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| Scan protocol |
Affymetrix protocol
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| Description |
matches column SPB80.6 in the RMA file
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| Data processing |
RMA expressionSet created using R/Bioconductor packages affy and limma; script available upon request
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| Submission date |
Oct 06, 2009 |
| Last update date |
Feb 16, 2010 |
| Contact name |
Stephan Emmerth |
| E-mail(s) |
marc.buehler@fmi.ch
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| Organization name |
FMI
|
| Lab |
Bühler
|
| Street address |
Maulbeerstrasse 66
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| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platform ID |
GPL7715 |
| Series (1) |
| GSE18440 |
Nuclear retention of fission yeast dicer is a prerequisite for RNAi-mediated heterochromatin assembly |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM459499.CEL.gz |
10.0 Mb |
(ftp)(http) |
CEL |
| Processed data are available on Series record |
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