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| Status |
Public on Jan 30, 2023 |
| Title |
PHYTOCHROME-INTERACTING FACTOR 7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChIP-Seq were performed using 35S::GFP-ASF1A, pHTR13::HTR13-HA, pHTR5::HTR5-HA, and pHTR5::HTR5-HA/pif7-1 transgenic plants after white light and 1 hr shade treatment. Two biological replicates were prepared for each genotype of plants grown under light and shade conditions. High-confidence binding peaks with cut off fold change > 1.5 and P-value <0.01. Our data reveal that ASF1 directly regulating a subset of target genes of PIF7. Furthermore, shade-elicited gene activation is accompanied by H3.3 enrichment, which is mediated by the PIF7-ASF1 regulatory module.
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| Overall design |
ChIP-seq was applied to comprehensively profile the ASF1A/H3.3/H3.1 landscape in response to shade exposure. 8-day-white-light grown seedlings were treated 1 hr shade before harvesting for pHTR13::HTR13-HA, pHTR5::HTR5-HA, pHTR5::HTR5-HA/pif7-1 DNA extraction using HA antibodies. 6-day-white-light grown seedlings were treated 1 hr shade before harvesting for 35S::GFP-ASF1A DNA extraction using GFP antibodies.
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| Contributor(s) |
Yang C, Li L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 19, 2022 |
| Last update date |
Jan 31, 2023 |
| Contact name |
Chuanwei Yang |
| E-mail(s) |
yangchuanwei@fudan.edu.cn
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| Organization name |
Fudan university
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| Department |
school of life sciences
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| Street address |
2005 Songhu Road, Yangpu District
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| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA798605 |
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