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Series GSE194005 Query DataSets for GSE194005
Status Public on Mar 01, 2022
Title Multimodal analyses of a non-human primate model harboring mutant amyloid precursor protein transgenes driven by the human EF1α promoter [WGS]
Organism Callithrix jacchus
Experiment type Other
Summary Alzheimer’s disease (AD) is the first leading cause of dementia which afflicts tens of millions of people worldwide. Despite many scientific progresses to dissect the molecular basis of AD from studies on various mouse models, it has been suffered from evolutionary species differences between mice and humans. Here, we report generation of a non-human primate, common marmoset (marmoset; Callithrix jacchus) model that ubiquitously expresses Amyloid-beta precursor protein (APP) transgenes with the Swedish (KM670/671NL) and Indiana (V717F) mutations. We generated two female transgenic marmosets (TG1 and TG2), whose transgene integration was thoroughly investigated by genomic PCR, whole genome sequencing (WGS) and Fluorescence in situ hybridization (FISH) analyses. Using the reprogramming technology, we confirmed the validity of the transgene expression in induced neurons in vitro. Moreover, we discovered structural changes in specific brain regions of transgenic marmosets by magnetic resonance imaging (MRI) analysis, including in the entorhinal cortex and hippocampus. In postmortem histological analysis, we detected increased Aβ plaque formation in the TG1 cerebrum at the age of 7 years, although evident neuronal loss or glial inflammation was not observed. Thus, this study summarizes our attempt to establish a non-human primate model of AD, which may be beneficial for drug development and further disease modeling in combination with other genetically engineered models.
 
Overall design Whole genome sequencing of fibroblasts derived from transgenic marmosets.
 
Contributor(s) Yoshimatsu S, Sanosaka T, Okano H
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Submission date Jan 19, 2022
Last update date Mar 01, 2022
Contact name Sho Yoshimatsu
E-mail(s) yoshima@a7.keio.jp
Organization name Keio University School of Medicine
Department Physiology
Lab Hideyuki Okano's Lab
Street address 35 Shinanomachi
City Shinjuku
State/province Tokyo
ZIP/Postal code 160-8582
Country Japan
 
Platforms (1)
GPL31253 DNBSEQ-G400 (Callithrix jacchus)
Samples (2)
GSM5825503 I655tgF
GSM5825504 I656tgF
This SubSeries is part of SuperSeries:
GSE194006 Multimodal analyses of a non-human primate model harboring mutant amyloid precursor protein transgenes driven by the human EF1α promoter
Relations
BioProject PRJNA798728

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Supplementary file Size Download File type/resource
GSE194005_Insertion_site.xlsx 9.0 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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